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. 2020 Jun 19;48(14):7640–7652. doi: 10.1093/nar/gkaa525

Table 5.

Photophysical properties of the sixteen 10 nt 2CNqA-ssDNAs and -dsDNAsa

ssDNA dsDNA
λ Abs λ Em ϵ ΦF ϵΦF <τ> k R k NR λ Abs λ Em ϵ ΦF ϵΦF <τ> k R k NR
NN [nm] [nm] [M−1 cm−1] [%] [M−1 cm−1] [ns] [107 s−1] [108 s−1] [nm] [nm] [M−1 cm−1] [%] [M−1 cm−1] [ns] [107 s−1] [108 s−1]
AA 357 452 10300 42 4300 11 3.8 0.53 355 453 9800 31 3000 9.5 3.3 0.73
AC 358 453 10400 35 3600 9.4 3.7 0.69 355 451 10100 29 2900 9.2 3.2 0.77
AG 358 453 10200 35 3500 8.6 4.0 0.76 356 454 9700 32 3100 9.7 3.3 0.70
AT 357 453 10600 18 1900 4.7 3.8 1.7 355 449 10000 32 3200 9.6 3.4 0.71
CA 358 457 9700 33 3200 8.6 3.9 0.78 356 458 8800 26 2300 8.5 3.0 0.87
CC 358 454 9500 27 2600 7.1 3.8 1.0 356 455 8700 28 2400 9.1 3.0 0.79
CG 359 453 9400 25 2400 6.2 4.1 1.2 356 459 8500 27 2300 9.2 3.0 0.79
CT 357 456 9700 15 1500 4.0 3.8 2.1 357 451 9000 31 2700 9.7 3.2 0.72
GA 358 453 9700 40 3900 11 3.8 0.56 355 455 9400 26 2400 8.5 3.0 0.87
GC 359 454 10100 28 2800 7.4 3.8 0.97 355 451 9500 25 2400 8.3 3.0 0.90
GG 358 453 9500 40 3800 9.9 4.0 0.61 357 456 9000 29 2700 9.2 3.2 0.77
GT 357 454 10000 17 1700 4.2 4.0 2.0 356 448 9700 29 2800 9.4 3.1 0.76
TA 358 456 9800 15 1400 3.7 4.0 2.3 356 456 9400 22 2100 7.4 3.0 1.0
TC 359 455 9600 13 1300 3.4 3.9 2.5 357 456 8800 26 2300 8.8 3.0 0.83
TG 360 453 9600 14 1300 3.3 4.2 2.7 358 459 8200 27 2200 8.9 3.0 0.82
TT 358 456 10000 10 1000 2.8 3.6 3.2 357 449 9200 29 2700 9.3 3.1 0.76

a λ Abs: absorption maximum, λEm: emission maximum, ϵ: molar absorptivity, ΦF: fluorescence quantum yield, ϵΦF: brightness, <τ>: average fluorescence lifetime, kR: radiative decay rate constant, kNR: non-radiative decay rate constant; Measured in 13 mM sodium phosphate buffer, 100 mM NaCl, pH 7.5 at RT. The ΦF was determined at λExc = 355 nm with quinine sulfate in 0.5 M H2SO4 (ΦF,R = 0.55) as the reference. The <τ> was determined at λExc = 377 nm and λEm = 446 nm. The reported ϵ, ΦF and <τ> values are the arithmetic mean of two or more measurements. Their standard errors of the mean (SEM) can be found in Supplementary Table S2. The reported kR and kNR values were calculated as described in Materials and Methods.