Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Jun 25;48(14):7748–7766. doi: 10.1093/nar/gkaa529

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Figure 5. — Epigenetic changes over transcriptionally altered genes in mESCs in serum and 2i. (A) Boxplots of average promoter 5mC in indicated cell lines over the total gene set, genes expressed under 2i conditions and genes repressed under 2i conditions. * indicates P < 0.05 (Wilcoxon test). (B) Examples of 5mC data at the promoters of the methylation regulated genes Dazl and Fkbp6 alongside RT-qPCR data for serum and 2i conditions. Promoter regions are marked by grey shadows. Barcharts show expression analysis of indicated genes by RT-qPCR, values represent mean ± SD of expression with respect to (wrt) TBP. * indicates P < 0.05 (unpaired t-test). (C) Scatter plot of fold change in expression 2i-serum (y-axis) versus fold change in average genic H3K27me3 levels (2i-serum) in WT (left), 3B3l (centre) and TKO (right) cells. Yellow = 2i induced genes, blue = 2i repressed genes. (D) Boxplot detailing expression changes serum to 2i (log2 fold changes) across WT, TKO, 3B3l, Eed⁻^/⁻ and EPZ6438 treated Dnmt1^tet/tetmESCs. Left: genes with overlapping H3K27me3 loss/expression gain in J1/TKO/3B3l mESCs. Right: genes with overlapping H3K27me3 gain/expression loss in J1/TKO/3B3l mESCs.