Figure 4.

Contribution of tRNA binding and catalytic activity by TruB to bacterial fitness. (A) Bacterial co-culture competition between E. coli wt and E. coli ΔtrmA (open circles), the ΔtrmA strain expressing TrmA wt protein (closed circles), TrmA F106E (open gray triangles), TrmA H125E (open inverted gray triangles) or TrmA C324A (diamonds). (B) Restriction enzyme and PCR-based bacterial co-culture competition assay between E. coli ΔtrmA expressing the catalytic inactive TrmA C324A variant and E. coli ΔtrmA expressing the tRNA binding variants TrmA F106E or H125E.