Table 2.

Kinetic and equilibrium binding constants for Tte riboswitch A14-variants

	k on × 104	S.E.a × 104	k off × 10−4	S.E. × 10−5	K D × 10−9	S.E. × 10−10	t 1/2
	M–1 s–1		s–1		M		min
Wildtype	11.60	1.13	2.12	1.6	1.84	0.8	54.5
2,6-Diaminopurine	2.07	0.40	2.51	3.5	12.3	7.9	46.0
2-Aminopurine	4.57	0.12	11.2	4.6	24.5	4.6	10.3
Purine	6.84	0.60	6.73	2.9	9.84	4.7	17.2

^aStandard error estimated using S.E. = S.D./sqrt(N), where N is the number of experiments.