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. 2020 Jun 25;48(14):8128–8145. doi: 10.1093/nar/gkaa541

Figure 2.

Figure 2.

The highly-conserved TBD-Pol2EXO interface stabilizes the Ctf18-1-8/Pol2CAT complex. (A) Molecular details of the TBD-Pol2EXO patch, showing backbone interactions in the left panel, and selected sidechain interactions in the right panel, with coloring as in Figure 1B. Alignments were performed in ClustalX2 (81) and displayed using ESPript 3.0 (82). Sc – Saccharomyces cerevisiae, Sp – Schizosaccharomyces pombe, Hs – Homo sapiens, Dm – Drosophila melanogaster, At – Arabidopsis thaliana. (B) EMSA supershift assay using 50 nM P1P2, 125 nM Pol2CAT and 125, 250 and 500 nM of the indicated Ctf18-1-8 variants.