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. 2020 Jun 25;48(14):8128–8145. doi: 10.1093/nar/gkaa541

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© The Author(s) 2020. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — Breaking the interaction between Pol ϵ and Ctf18-RFC does not affect chromosome cohesion. (A, B) Analysis of chromosome cohesion. Cells carrying a tetO at the URA3 locus (57), a TetR-GFP and a SPC42-mCHERRY allele were arrested in G1 and synchronously released in S phase and collected every 15 minutes. The distance between the Spc42-mCHERRY was scored and cells with spindles 1–2.5 μm were analyzed for number of GFP foci present. (A) Example of the images analyzed. (B) Summary of the results of three experiments. For each experiment and strain, around 400 cells with spindles 1–2.5 μm were analyzed. The average and standard deviation of three independent experiments are shown. (C) ctf18-RAA pol2-5A/dcc1WH3Δ are not sensitive to Benomyl nor show synthetic defects with mad2Δ. The strains were diluted 1:10 and spotted on the indicated medium. (D) Analysis of the meiotic progeny of the diploid strains indicated. Plates were scanned after 3 days growth at 24°C.