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. 2020 Jul 1;48(14):7818–7833. doi: 10.1093/nar/gkaa555

Figure 4.

Figure 4.

The N- and C-terminus of BRCA2 are prime regions for BRCA2 self-interactions. (A) Constructs of wild type and truncated mutant BRCA2 proteins tested in pulldown interaction assay shown in B. (B) Scheme of protein interaction pulldown assay. (C) Protein interactions between 0.65 nM full-length BRCA2 (GST-BRCA2) and increasing amount of BRCA2 N-terminus (MBP-BRCA2NT) at 0.5, 1.5, 3, 5 and 6 nM. (D) Protein interactions between 30 nM GST-tagged N-terminus of BRCA2 (GST-BRCA2NT) and MBP-tagged C-terminus of BRCA2 (MBP-BRCA2CT) at 30, 100 and 200 nM. (E) Protein interactions between 30 nM GST-tagged N-terminus of BRCA2 (GST-BRCA2NT) and MBP-tagged N-terminus of BRCA2 (MBP-BRCA2NT) at 30 nM, 100 nM and 200 nM. Asterisks in D and E indicate degraded GST-BRCA2NT fragments.