(A) MCh induced seizures at lower concentrations in older Kcna1−/− mice. (B) MCh concentration response curves exhibited a leftward shift in SD90 Kcna1−/− mice for inspiratory time (Ti), (C) expiratory time (Te), and (D) and respiration rate (f). This resulted in significantly reduced MCh EC50’s (right). Significance was determined by a one-way ANOVA followed by Tukey’s multiple comparisons test, **
p < 0.01, ***
p < 0.001 v. P32-36 and $$
p < 0.01, $$$
p < 0.001 v. P42-46, n = 5-6.