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. Author manuscript; available in PMC: 2020 Nov 4.
Published in final edited form as: Epilepsia. 2018 Jan 12;59(2):345–357. doi: 10.1111/epi.13971

Figure 6. Kcna1 mRNA and protein is not present in lung, and does not participate in airway smooth muscle reactivity to MCh.

Figure 6

Kcna1 (A) mRNA and (B) protein were expressed to ~30-40% of wild-type levels in Kcna1+/− brain tissue and absent in Kcna1−/− mice brains. In contrast, Kcna1 mRNA and protein were not detected in lung tissue from all three genotypes. Significance was determined by a two-way ANOVA for tissue and genotype followed by Tukey’s multiple comparisons test, *** p < 0.001 v. Kcna1+/+ and $ p < 0.05, $$ p < 0.01 v. Kcna1+/−, n = 3 per genotype. (C) Concentration-response curves demonstrating the maximal contractile response and the sensitivity to MCh of isolated trachea did not differ between Kcna1+/+ and Kcna1−/− mice (n = 4). Inset, concentration response curves normalized to the maximum contraction for each genotype. Significance was determined by a two-way ANOVA repeated measures for MCh concentration and genotype followed by Tukey’s multiple comparisons test.