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. 2020 Nov 4;15(11):e0241867. doi: 10.1371/journal.pone.0241867

Fig 2. Construction of Cas9-mediated S. oneidensis flaG gene deletion mutants.

Fig 2

(A): The editing schematic diagram and screening primers are shown for deletion of the flaG gene. (B): The phenotype of different S. oneidensis strains. WT, wild type. Cas9, the wild type strain harboring Cre, Cas9, RecET and AprR in the chromosome. Cas9+sgRNA, the wild type strain harboring donor template, sgRNA, KanR, Cas9, RecET and AprR in the chromosome. (C): PCR screening of the initial transconjugant colonies. L: DNA ladder. WT: wild type as positive control. 1–16: 16 randomly picked colonies.