Table 1. Summary of successful genome editing in four different bacteria species.
| Strain ID (landing pad insertion site (strand)) | Targeted gene/pathwaya | gRNA sequence | Editing type (size, chromosome locationb) |
|---|---|---|---|
| Aeromonas salmonicida subsp. Pectinolytica 34mel, (3403527–3403528 (-)) | hpd (locus_tag: Asalp_21790) | AGAACCCAGCGATGCGTAAC | Deletion (1512 bp, 2343500–2345011) |
| Pseudomonas simiae WCS417r, (1774856–1774,857 (-)) | Thioesterase (locus_tag: PS417_19550) | GCATACAGCGTGTTTCAGTT | Deletion (520 bp, 4252971–4253490) |
| Shewanella oneidensis MR-1, (3069441-3069442(+)) | flaG (locus_tag: SO_3236) | ACAAATGCTTATGATGGTGAGGTATAGCTA | Deletion (909 bp, 3375174–3376082) |
| Photorhabdus luminescens subsp. laumondii TT01, (4881822–4881823 (+)) | NRPSc_1 (locus_tag: PluTT01m_04610) | TGCAGATTTATATTAATAGC | Deletion (3294 bp, 1020927–1024220) |
| NRPS_2 (locus_tag: PluTT01m_05670) | TAAATAGTTATGATAACTAT | Deletion (2300 bp, 1276716–1279015) | |
| NRPS_3 (locus_tag: PluTT01m_06165–06180) | GTATACATATCCAAGTTACT | Deletion (7705 bp, 1391134–1398838) | |
| NRPS_4 (locus_tag: PluTT01m_09665–09670) | ATCGAGATATTCTTATTTAT | Deletion (5117 bp, 2235387–2240503) | |
| NRPS_5 (locus_tag: PluTT01m_11950–11970) | CCCTATTCCGTTAGCCCTGA | Deletion (8003 bp, 2713290–2721292) | |
| NRPS_6 (locus_tag: PluTT01m_13700) | TACCCGTTTTTATTTCACCG | Deletion (7011 bp, 3166873–3173883) | |
| NRPS_7 (locus_tag: PluTT01m_16070) | AGTGCCGTTCCGTCATCTGG | Deletion (6034 bp, 3656505–3662538) | |
| NRPS_9 (locus_tag: PluTT01m_16105) | CGGTATCTAAGTCTCTTGGA | Deletion (5066 bp, 3683630–3688695) | |
| NRPS_10 (locus_tag: PluTT01m_16815) | CCAAGAGAATGTGCTGTCTG | Deletion (6067 bp, 3875195–3881261) | |
| NRPS_11 (locus_tag: PluTT01m_18130–18145) | AAACCTGTATGTATCGGGAG | Deletion (6188 bp, 4162085–4168272) |
a: The gRNA targeting site might be in the upstream regulatory region or in the open reading frame.
b: the position of the start and the end of the deletion on the chromosome.
c: NRPS stands for non-ribosomal peptide/polyketide synthase gene cluster.