Table 3.

Prediction of the mutational effects on the structural stability.

Protein	Amino Acid Changes	SVM2 Prediction Effect	DDG Value (kcal/mol)
ORF1a Polyprotein	I300F	Decrease	−1.79
ORF1a Polyprotein	N2155S	Decrease	−0.60
ORF1a Polyprotein	Q2702H	Decrease	−0.68
ORF1a Polyprotein	D3355G	Decrease	−0.95
ORF1a Polyprotein	K3353R	Increase	−0.13
ORF1a Polyprotein	V3572M	Decrease	−0.88
ORF1a Polyprotein	P3952S	Decrease	−1.21
ORF1b Polyprotein	P214L	Decrease	−0.83
ORF1b Polyprotein	E1084D	Decrease	−0.75
ORF1b Polyprotein	V1883T	Decrease	−1.46
Surface Glycoprotein	D623G	Decrease	−0.93
Surface Glycoprotein	F1118L	Decrease	−0.81
ORF3a protein	Q38L	Increase	0.12
ORF3a protein	S40L	Increase	0.40
ORF3a protein	Q57H	Decrease	−0.90
ORF3a protein	G172C	Decrease	−0.83
ORF6 protein	W27L	Decrease	−0.96
ORF8	A65V	Increase	0.02
ORF8	L84S	Decrease	−2.29
Neucleocapsid phospoprotein	S202N	Increase	−0.78
Neucleocapsid phospoprotein	R203K	Decrease	−0.93
Neucleocapsid phospoprotein	G204R	Decrease	−0.52
Neucleocapsid phospoprotein	K373N	Increase	−0.10
Neucleocapsid phospoprotein	D377G	Decrease	−0.44