Figure 3.

LY294002 and idelalisib suppress Snail and β-catenin expressions. (A, B) The mRNA levels of Snail and β-catenin were quantified by qRT-PCR following treatment with 20 μM LY294002 or idelalisib in Huh-BAT and HepG2 cells. (C, D) Huh-BAT cells and HepG2 cells were treated with 20 μM LY294002 or idelalisib, for various time points. The protein expression levels of Snail were evaluated by immunoblotting. β-actin was used as a loading control. (E, F) Huh-BAT cells and HepG2 cells were treated with 20 μM LY294002 or idelalisib, for various time points. The protein expression levels of Snail and phospho-GSK-3β were evaluated by immunoblotting. β-actin was used as a loading control. Data are presented as the mean±standard deviation. ^*P<0.05 and ^†P<0.01 compared with untreated control cells. P-GSK, phospho-glycogen synthase kinase; qRT-PCR, quantitative Real-time polymerase chain reaction.