Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Gene (Magnetococcus marinus MC-1) | NavMs | Uniprot | Mmc1_0789 | A0L5S6_MAGMM |
| Strain, strain background (Escherichia coli) | Over Express C41 (DE3) | Sigma-Aldrich | CMC0017 | Chemically competent E. coli for expression of toxic proteins |
| Cell line (Cricetulus griseus) | CHO-K1 | CedarLine Laboratories | RRID:CVCL_0214 | |
| Recombinant DNA reagent | pET15b plasmid encoding NavMs | PMID:28205548 | Plasmid for NavMs and NavMsL expression for structural study | |
| Recombinant DNA reagent | His-NavMs | addgene.org | 100004 | pTracer-CMV2, IRES GFP plasmid encoding NavMs |
| Sequence-based reagent | F208L_F | This Paper | PCR Primer for NavMsL (forward) | 5'-CTCACCACCCTGA
CCGTGCTCAACCTGT TTATTGG-3′ |
| Squence-based reagent | F208L_R | This Paper | PCR Primer for NavMsL (reverse) | 5′-GAGCACGGTCAGG
GTGGTGAGCATGATG +AACGGGATG-3′ |
| Chemical compound, drug | Cannabidiol (CBD) | Sigma-Aldrich | C7515 | |
| Chemical compound, drug | Cannabidiol (CBD) | Toronto Research Chemicals | F175300 | |
| Software, algorithm | XDS | PMID:20124692 | RRID:SCR_015652 | Data Processing |
| Software, algorithm | Aimless | doi:10.1107/S0907444913000061 | RRID:SCR_015747 | Data Processing |
| Software, algorithm | CCP4 | PMID:15299374 | RRID:SCR_007255 | Structure Determination/Refinement |
| Software, algorithm | Phaser | PMID:19461840 | RRID:SCR_014219 | Structure Determination/Refinement |
| Software, algorithm | Coot | PMID:20383002 | RRID:SCR_014222 | Structure Determination/Refinement |
| Software, algorithm | REFMAC | PMID:21460454 | RRID:SCR_014225 | Structure Determination/Refinement |
| Software, algorithm | PROCHECK | doi:10.1107/s0021889892009944 | RRID:SCR_019043 | Structure Determination/Refinement |
| Software, algorithm | Molprobity | PMID:29067766 | RRID:SCR_014226 | Structure Determination/Refinement |
| Software, algorithm | BUSTER | PMID:22505257 | RRID:SCR_015653 | Structure Determination/Refinement |
| Software, algorithm | CCP4mg | PMID:21460457 | RRID:SCR_019041 | Structure Presentation |
| Software, algorithm | PatchMaster | HEKA Elektronik | RRID:SCR_000034 | Data Acquisition |
| Software, algorithm | FitMaster | HEKA Elektronik | RRID:SCR_016233 | Data Analysis |
| Software, algorithm | IGOR Pro | Wavemetrics, Lake Oswego, OR | RRID:SCR_000325 | Data Analysis |
| Software, algorithm | Clustal Omega | PMID:24170397 | RRID:SCR_001591 | Sequence Alignment |
| Software, algorithm | HOLE | PMID:9195488 | www.holeprogram.org | Channel Pore Dimension Analysis |