Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Gene (Mus musculus) | Epo | GenBank | NM_007942.2 | Erythropoietin |
| Gene (Mus musculus) | Arid1a | GenBank | NM_001080819.2 | Arid1a |
| Gene (Mus musculus) | Ctnnb1 | GenBank | NM_007614.3 | Beta-catenin |
| Gene (Mus musculus) | Apc | GenBank | NM_001360980.1 | Adenomatous polyposis coli |
| Strain, strain background (Mus musculus) | Arid1a-lox | From Z. Wang’s lab | Arid1atm1.1Zhwa/J | https://www.jax.org/strain/027717 |
| Strain, strain background (Mus musculus) | Apc-lox | From Perret-Colnot’s lab | Apctm2.1Cip | https://www.infrafrontier.eu/search?keyword=EM:05566 |
| Strain, strain background (Mus musculus) | Ttr-Cre-Tam | From Perret-Colnot’s lab | Tg(Ttr-cre/Esr1*)1Vco | https://www.infrafrontier.eu/search?keyword=EM:01713 |
| Genetic reagent (Adenovirus 5) | Ad-Cre | Université de Nantes, France | Ad5-CAG-Cre | https://umr1089.univ-nantes.fr/facilities-cores/cpv/translational-vector-core-2201753.kjsp?RH=1519296751975 |
| Cell line (Mus musculus) | Mouse hepatoma | From Christine Perret’s lab | Hepa 1-6 [Hepa1-6] (ATCC CRL-1830) | For transfection experiments |
| Antibody | anti-Arid1a (Rabbit monoclonal) | Abcam | Cat# 182560 [EPR13501] |
IHC(1:1000), WB (1:2000) |
| Antibody | anti-Glul (GS) (Mouse monoclonal) | BD Biosciences | Cat# 610518, RRID:AB_397880 | IHC(1:400), WB (1:5000) |
| Antibody | anti-HBB (Mouse monoclonal) | Proteintech | Cat# 16216–1-AP, RRID:AB_10598329 | IHC(1:200), WB (1:2000) |
| Antibody | anti-HIF1α (Rabbit polyclonal) | Novus | Cat# NB100-449, RRID:AB_10001045 | WB nuclear extract (1:500) |
| Antibody | anti-HIF2α (Rabbit polyclonal) | Novus | Cat# NB100-122, RRID:AB_10002593 | WB nuclear extract (1:500) |
| Antibody | Anti-Tcf4 (Tcf7l2) (Mouse monoclonal) | Millipore | Cat# 05–511, RRID:AB_309772 | ChIP: 3 μg |
| Antibody | Anti-H3K27Ac (Rabbit polyclonal) | Active Motif | Cat# 39133, RRID:AB_2561016 | ChIP: 3 μg |
| Antibody | Anti-H3K27me3 (Rabbit polyclonal) | Active Motif | Cat# 39155, RRID:AB_2561020 | ChIP: 3 μg |
| Antibody | IgG (Mouse) | Thermo Fisher Scientific | Cat# 10400C, RRID:AB_2532980 | ChIP: 3 μg |
| Antibody | Anti-CD71-FITC (Rat monoclonal) | BD Biosciences | Cat# 553266, RRID:AB_394743 | FACS (1:100) |
| Antibody | Anti-Ter119-PE (rat monoclonal) | BD Biosciences | Cat# 553673, RRID:AB_394986 | FACS (1:100) |
| Antibody | Anti-β-actin (mouse monoclonal) | Sigma-Aldrich | Cat# A5441, RRID:AB_476744 | WB (1:10000) |
| Antibody | Anti-lamin A/C (rabbit polyclonal) | Cell Signaling Technology | Cat# 2032, RRID:AB_2136278 | WB nuclear extract (1:500) |
| Antibody | IgG, HRP-conjugated (horse, anti-mouse) | Cell Signaling Technology | Cat# 7076, RRID:AB_330924 | WB (1:2000) |
| Antibody | IgG, HRP-conjugated (goat, anti-rabbit) | Cell Signaling Technology | Cat# 7074, RRID:AB_2099233 | WB (1:2000) |
| Antibody | IgG, biotinylated (goat, anti-rabbit) | Vector lab | Cat# BA-1000, RRID:AB_2313606 | IHC (1:200) |
| Commercial assay or kit | MOM mouse on mouse | Vector Laboratories | Cat# BMK-2202, RRID:AB_2336833 | Kit |
| Sequence-based reagent | 18S | Thermo Fisher Scientific | Taqman Assay 4308329 | qPCR primers |
| Sequence-based reagent | Glul | Thermo Fisher Scientific | Taqman Assay Mm00725701_si | qPCR primers Mus musculus |
| Sequence-based reagent | Axin2 | Thermo Fisher Scientific | Taqman Assay Mm00443610_m1 | qPCR primers Mus musculus |
| Sequence-based reagent | Arid1a (total) | Thermo Fisher Scientific | Taqman Assay Mm00473838_m1 | qPCR primers Mus musculus |
| Sequence-based reagent | Arid1a (not excised by Cre) | Thermo Fisher Scientific | Taqman Assay Mm00473841_m1 | qPCR primers Mus musculus |
| Sequence-based reagent | Apc (total) | Thermo Fisher Scientific | Taqman Assay Mm00545877_m1 | qPCR primers Mus musculus |
| Sequence-based reagent | Apc (not excised by Cre) | Thermo Fisher Scientific | Taqman Assay Mm01130462_m1 | qPCR primers Mus musculus |
| Sequence-based reagent | Epo | Thermo Fisher Scientific | Taqman Assay Mm01202755_m1 | qPCR primers Mus musculus |
| Sequence- based reagent |
18 s | Eurogentec | F_GTAACCCGTTGAACCCCATT R_CCATCCAATCGGTAGCG |
SybrGreen qPCR primers |
| Sequence-based reagent | Angiopoietin-like 2 (Angptl2) | Eurogentec | F_CCGCAACATGAACTCGAGAG R_GTGCTCCAGGTCCTTGTACT |
SybrGreen qPCR primers Mus musculus |
| Sequence-based reagent | Carbonic anhydrase 9 (Car9) | Eurogentec | F_GACCTCGTGATTCTCGGCTA R_GAGAAGGCCAAACACCAAGG |
SybrGreen qPCR primers Mus musculus |
| Sequence-based reagent | Cyclin D1 (Ccnd1) | Eurogentec | F_AGAAGTGCGAAGAGGAGGTC R_TTCTCGGCAGTCAAGGGAAT |
SybrGreen qPCR primers Mus musculus |
| Sequence-based reagent | Enolase 2, gamma neuronal (Eno2) | Eurogentec | F_TGGATTTCAAGTCTCCCGCT R_TCAGGTCATCGCCCACTATC |
SybrGreen qPCR primers Mus musculus |
| Sequence-based reagent | Erythropoietin receptor (Epo-r) | Eurogentec | F_ATGACTTTCGTGACTCACCCT R_GGGCTCCGAAGAACTTCTGTG |
SybrGreen qPCR primers Mus musculus |
| Sequence-based reagent | FMS-like tyrosine kinase 1 (Flt1) | Eurogentec | F_AGAGGAGGATGAGGGTGTCT R_GGGAACTTCATCTGGGTCCA |
SybrGreen qPCR primers Mus musculus |
| Sequence-based reagent | GATA binding protein 1 (Gata1) | Eurogentec | F_TTCCCACTACTGCTGCTACC R_GCGGCCTCTATTTCAAGCTC |
SybrGreen qPCR primers Mus musculus |
| Sequence-based reagent | GATA binding protein 2 (Gata2) | Eurogentec | F_GCCGGTTCTGTCCATTCATC R_ATGGCAGCAGTCTCTTCCAT |
SybrGreen qPCR primers Mus musculus |
| Sequence-based reagent | Inhibin beta-B (Inhbb) | Eurogentec | F_GTACCTGAAACTGCTCCCCT R_ATGGCCTCTGTGATGGGAAA |
SybrGreen qPCR primers Mus musculus |
| Sequence-based reagent | Potassium channel tetramer domain contain. 11 (Kctd11) | Eurogentec | F_TGACTTCTACCAGATCCGGC R_TCAGGGTCAGTGCAGAAGAG |
SybrGreen qPCR primers Mus musculus |
| Sequence- based reagent |
Kinase insert domain protein receptor (Kdr) | Eurogentec | F_AGAAGATGCCCATGACCCAA R_TCACCCATCCTCAACACACA |
SybrGreen qPCR primers Mus musculus |
| Sequence-based reagent | Nuclear factor, erythroid derived 2 (Nfe2) | Eurogentec | F_GATGTCCCGAACTAGAGCCA R_ACACCCTTGGCCTTAGAGTC |
SybrGreen qPCR primers Mus musculus |
| Sequence-based reagent | Platelet derived growth factor receptor, alpha polypeptide (Pdgfra) | Eurogentec | F_ACAGCTCACAGACTTCGGAA R_AGAAGATGATACCCGGAGCG |
SybrGreen qPCR primers Mus musculus |
| Sequence-based reagent | Phosphoglycerate kinase 1 (Pgk1) | Eurogentec | F_TGGCACCAGGAACCCTTAAA R_AGCTCAGCCTTTACAGCTCA |
SybrGreen qPCR primers Mus musculus |
| Sequence-based reagent | Placenta-specific 8 (Plac8) | Eurogentec | F_TGATTGCTTCAGTGACTGCG R_GTTCATGGCTCTCCTCCTGT |
SybrGreen qPCR primers Mus musculus |
| Sequence-based reagent | Protein tyrosine phosphatase, receptor type, B (Ptprb) | Eurogentec | F_TGGACCCTGGGATCTAAGGA R_GTGGTCACTGCAAGCTTCAA |
SybrGreen qPCR primers Mus musculus |
| Sequence-based reagent | Member RAS oncogene family (Rab42) | Eurogentec | F_GGCGTTCTGTTGGTCTTTGA R_GCAAGTTCCTCTGCTTCCTG |
SybrGreen qPCR primers Mus musculus |
| Sequence-based reagent | Vascular endothelial growth factor A (Vegfa) | Eurogentec | F_GCTGTAACGATGAAGCCCTG R_CGCTCCAGGATTTAAACCGG |
SybrGreen qPCR primers Mus musculus |
| Sequence-based reagent | Zinc finger protein, multitype 1 (Zfpm1) | Eurogentec | F_CCTTGAGATGGCGTTCACAG R_CCTGCTCTACTACTGTGCCA |
SybrGreen qPCR primers Mus musculus |
| Sequence-based reagent | AT-rich interaction domain 1A (ARID1A) | Eurogentec | F_AAGCCACCAACTCCAGCATCCA R_CGCTTCTGGAATGTGGAGTCAC |
SybrGreen qPCR primers (Homo sapiens) |
| Sequence-based reagent | Adenomatous polyposis coli (APC) | Eurogentec | F_CACACTTCCAACTTCTCGCAACG R_AGGCTGCATGAGAGCACTTGTG |
SybrGreen qPCR primers (Homo sapiens) |
| Sequence-based reagent | Erythropoietin (EPO) | Eurogentec | F_GCATGTGGATAAAGCCGTCAGTG R_GAGTTTGCGGAAAGTGTCAGCAG |
SybrGreen qPCR primers (Homo sapiens) |
| Sequence-based reagent | DOS7-binding site (Control) | Eurogentec | F_GGGGTAGGAACCAATGAAA R_TTTCATTGGTTCCTACCCC |
EMSA probe Mus musculus |
| Sequence-based reagent | HNF4-responsive element (DR2) | Eurogentec | F_GCCCGGCTGACCTCTTGACCCCTCTGGGCTTGAG R_CTCAAGCCCAGAGGGGTCAAGAGGTCAGCCGGGC |
EMSA probe Mus musculus |
| Sequence-based reagent | Wnt-reponsive element | Eurogentec | F_CATCCCCCTTTGATCTTACC R_GGTAAGATCAAAGGGGGATG |
EMSA probe |
| Sequence- based reagent |
Negative control region | Eurogentec | F_ACACACCTTGAATCCCGT R_CCCAGCTAGAATGAACAAG |
qPCR primers for ChIP and ATAC |
| Sequence-based reagent | Hepatic Epo 3’ enhancer | Eurogentec | F_CTGTACCTCACCCCATCTGGTC R_CCCAGCTCACTCAGCACTTGTCC |
qPCR primers for ChIP and ATAC |
| Sequence-based reagent | EPO-enh-5’ (1) | Eurogentec | F_GGCAACAGCTGAAATCACCAA R_TCCCAGATCTGATGCCTTGC |
qPCR primers for ATAC |
| Sequence-based reagent | EPO-enhHIF (2) | Eurogentec | F_CTGTACCTCACCCCATCTGG R_CAGAGGGGTCAAGAGGTCAG |
qPCR primers for ChIP and ATAC |
| Sequence-based reagent | EPO-enhHnf4 (3) | Eurogentec | F_GCAAGGCATCAGATCTGGGA R_AGACAGCCTTGAATGGAGCC |
qPCR primers for ChIP and ATAC |