Table 1.
Top 20 proteins significantly over-produced (left hand side) or under-produced (right hand side) in C. jejuni cells that strained brightly with RSG after exposure to 100 × MIC of penicillin G for 24 h.
| Significantly over-produced proteins | Significantly under-produced proteins | ||||||
|---|---|---|---|---|---|---|---|
| Locus | Gene name | Protein name | Fold change | Locus | Gene name | Protein name | Fold change |
| Cj0755 | cfrA | Ferric enterobactin uptake receptor | 12.4733 | Cj1430c | rfbC | dTDP-4-dehydrorhamnose 3,5-epimerase | ∞ |
| Cj1039 | murG | UDP-N-acetylglucosamine–N-acetylmuramyl-(pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase | 7.8637 | Cj1567c | nuoM | NADH-quinone oxidoreductase I subunit M | 0.0225 |
| Cj0367c | cmeA | Multidrug efflux pump protein CmeA | 4.7408 | Cj1685c | bioB | Biotin synthase | 0.0282 |
| Cj0926 | Membrane protein | 4.1717 | Cj1378 | selA | L-seryl-tRNA(Sec) selenium transferase | 0.0415 | |
| Cj0365c | cmeC | Multidrug efflux pump protein CmeC | 3.9992 | Cj0117 | pfs | Aminodeoxyfutalosine nucleosidase | 0.056 |
| Cj1357c | nrfA | Cytochrome c nitrite reductase cytochrome c552 subunit | 3.8977 | Cj0314 | lysA | Diaminopimelate decarboxylase | 0.0587 |
| Cj0318 | fliF | Flagellar MS-ring protein | 3.7812 | Cj0002 | dnaN | DNA polymerase III subunit beta | 0.0627 |
| Cj0946 | Lipoprotein | 3.6892 | Cj1725 | Periplasmic protein | 0.0859 | ||
| Cj0842 | Lipoprotein | 3.6419 | Cj1171c | ppi | Peptidyl-prolyl cis-trans isomerase | 0.0899 | |
| Cj0329c | plsX | Phosphate acyltransferase | 3.4946 | Cj1315c | hisH | Imidazole glycerol phosphate synthase subunit HisH | 0.0975 |
| Cj1215 | Peptidase M23 family protein | 3.4565 | Cj1720 | Hypothetical protein Cj1720 | 0.1062 | ||
| Cj0277 | mreC | Rod shape-determining protein MreC | 3.3915 | Cj0405 | aroE | Shikimate 5-dehydrogenase | 0.1123 |
| Cj1573c | nuoG | NADH-quinone oxidoreductase subunit G | 3.3741 | Cj1041c | ATP/GTP-binding protein | 0.1129 | |
| Cj0508 | pbpA | Penicillin G-binding protein | 3.2216 | Cj1250 | purD | Phosphoribosylamine–glycine ligase | 0.1131 |
| Cj0151c | Periplasmic protein | 3.1399 | Cj1670c | cgpA | Glycoprotein CpgA | 0.1286 | |
| Cj1207c | Lipoprotein thiredoxin | 3.1258 | Cj1601 | hisA | 1-(5-phosphoribosyl)-5 | 0.1415 | |
| Cj0268c | Transmembrane protein | 3.1233 | Cj0440c | Transcriptional regulator | 0.1449 | ||
| Cj0734c | hisJ | Histidine-binding protein | 3.0803 | Cj0234c | frr | Ribosome recycling factor | 0.1472 |
| Cj0090 | Lipoprotein | 3.0552 | Cj1516 | Oxidoreductase | 0.158 | ||
| Cj0853c | hemL | Glutamate-1-semialdehyde aminotransferase | 2.8635 | Cj0014c | Integral membrane protein | 0.159 | |
The proteome of these cells was compared to the proteome of a control culture not exposed to antibiotic. The proteins listed showed an abundance change of 2-fold or more and the q value was < 0.05.