Figure 4.

FhKTM-specific cytokine immune responses in BALB/c mice after vaccination. At 0, 4, and 24 dpi, spleens were collected and splenocytes were stimulated in vitro with FhKTM for 72 h. The culture supernatants from spleen cells were assessed for the production of IL-17A, IFN-γ, IL-10, IL-5, and IL-4 by ELISA. Results are shown as mean ± SD and levels of significance as indicated by p-values and are representative of two or three independent experiments. They were assessed by one-way ANOVA and Tukey's multiple comparison tests *p < 0.05; **p < 0.01.