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. 2020 Oct 28;2020:3921897. doi: 10.1155/2020/3921897

Figure 5.

Figure 5

HS activates Sgk1/Nedd4-2 via BMP4 and p38 MAPK signaling. (a) Representative Western blots and summarized data showing BMP4 protein levels in endothelial cells isolated from SS rats either under control conditions or treated with an additional 40 mM NaCl in the culture medium for 24 hours. The data are the means ± SEMs of 6 experiments in each group. ∗indicates P < 0.05vs. control. (b) Representative Western blots and summarized data showing p38 MAPK and p-p38 MAPK protein levels under control conditions or treated with HS additional 40 mM NaCl in the culture medium for 24 hours, in the absence of and in the presence of 100 ng/mL noggin. ∗indicates P < 0.05vs. control; #represents P < 0.05vs. HS (n = 6 for each group). (c–d) Representative Western blots and summarized data showing Sgk1, p-Sgk1, Nedd4-2, and p-Nedd4-2 protein levels under control conditions or treated with additional 40 mM NaCl in the culture medium for 24 hours, in the absence of and in the presence of either 100 ng/mL noggin or 10 μM SB202190 for 24 hours. ∗indicates P < 0.05vs. control; #represents P < 0.05vs. HS (n = 6 for each group). (e–h) Representative Western blots and summarized data showing BMP4, p-p38 MAPK, p38 MAPK, p-Sgk1, Sgk1, p-Nedd4-2, and Nedd4-2 protein levels in primary cultured endothelial cells isolated from SS rats either under control conditions or treated with an additional 80 mM mannitol in the culture medium for 24 hours. The data are the means ± SEMs of 6 experiments in each group.