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. 2020 Oct 27;2020:8704146. doi: 10.1155/2020/8704146

Figure 2.

Figure 2

Effects of isoorientin on the phosphorylation of GSK3β (Ser9) and TNF-α in LPS-induced RAW264.7 cells. (a) Effects of isoorientin and LiCl on p-GSK3β and (b) effects of MK-2206 on p-GSK3β. RAW264.7 cells were treated with isoorientin (6.25 and 25 μM) or LiCl (100 μM) for 1 h without (a) or with (b) prior treatment of MK-2206 (1 μM) for 30 min and then stimulated with LPS (50 ng/mL). After 30 min of LPS stimulation, the cells were collected for immunoblotting of p-GSK3β, GSK3β, and GAPDH (n = 3, 3 independent experiments). After 8 h of LPS stimulation, the cell supernatants were collected, and the levels of TNF-α were detected by ELISA (n = 4, 4 replicates) (c). The data were expressed as mean ± SEM. Statistical analysis was done using ANOVA. ∗P < 0.05 versus the control group, #P < 0.05 versus the LPS group. ∗∗P < 0.05 versus the isoorientin 6.25 μM group, ##P < 0.05 versus the isoorientin 25 μM group.