Figure 5.

Effects of isoorientin on ZO-1 (a), occludin (a), and p-GSK3β (b) in the brain of endotoxemia mice. Isoorientin (25 and 50 mg/kg, ig) and LiCl (100 mg/kg, ig) were given once a day for 5 days, and after 30 min of the last administration, LPS (5 mg/kg, ip) was given. The brain tissues were collected after 6 h of LPS injection. Western blotting was used to measure the expression of ZO-1, occludin, p-GSK3β, and GSK3β. The data were expressed as mean ± SEM (n = 3, 3 biological replicates). Statistical analysis was done using ANOVA. ^∗P < 0.05 versus the control group, ^#P < 0.05 versus the LPS group.