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. 2020 Sep 30;48(19):10820–10831. doi: 10.1093/nar/gkaa799

Figure 3.

Figure 3.

Single-molecule tracking of fluorescent Nhp6A along DNA bound by non-fluorescent HU. (A) Single-molecule traces of Nhp6A in the presence of different concentrations of HU. (B) MSD plots of Nhp6A with 0–32 nM HU. (C) Fis concentration dependence of average diffusion coefficients (D) for Nhp6A. (D) HU concentration dependence of average travel distances of Nhp6A at 0.4 s intervals (<Δx>, black squares) and average spacings between neighboring HU molecules (<d>, green circles). (E) Displacement distributions of Nhp6A at 0.176 s intervals with 0–32 nM HU. Black-dashed curves are the best fitted curves with double Gaussian functions. Red and blue curves are the best fitted curve of the high-mobility and low-mobility modes, respectively. (F) HU concentration dependence of diffusion coefficients in two modes of Nhp6A. Red circles and blue triangles represent the diffusion coefficients of the high-mobility and low-mobility modes, respectively. (G) HU concentration dependence of the fraction of the low-mobility mode of Nhp6A. The error bars in panels B, C, D, F, and G are described in the caption of Figure 2.