Figure 4.
(A) Induction of 2-nt, 3-nt, 4-nt or 5-nt bulge-loops in the RNA (blue) upon hybridization with the peptidyl-oligonucleotide conjugates (green), which become susceptible to cleavage by closely juxtaposed peptide (scissors). (B and C) Cleavage patterns of 3′-FITC-tRNAPhe observed upon treatment with α- and β- series of Type 2 conjugates, summarizing data shown in Figure 3. Arrows indicate the point of cleavage. Major cleavage sites are presented with solid line arrows, whereas minor cleavage sites are indicated with dotted arrows. (B) Identical cleavage patterns of 3′-FITC-tRNAPhe were witnessed for BC-2α and BC-2β, as well as for BC5L-α and BC5L-β conjugates. (C) Differences in cleavage patterns were witnessed for BC3/4-α conjugates (black arrows) versus BC3/4-β conjugates (red arrows), as well as for BC5-α conjugates against BC5-β conjugates.