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. 2020 Oct 6;48(19):10973–10985. doi: 10.1093/nar/gkaa795

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© The Author(s) 2020. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — Behavioural changes imply neuronal defects in csb-1 mutants. (A) Pharyngeal pumping in wt and csb-1 mutants upon UVB irradiation. Nematodes were irradiated at Day 1 of adulthood, and assayed 24 h after irradiation (Day 2), 48 h later (Day 3) and 72 h later (Day 4) (n > 15 per group). (B) Pharyngeal pumping measured in the wt, the csb-1 mutant and the csb-1(ok2335);CSB-1::GFP rescue line upon UVB irradiation. Nematodes were irradiated at Day 1 of adulthood, and assayed 48 h after irradiation (n > 13 per group). (C) Pharyngeal pumping in the wt and csb-1(ok2335) (each carrying the p_mec-4GFP neuronal reporter) and the csb-1(ok2335);CSB-1::GFP rescue line upon Illudin M treatment at different concentrations. Whiskers in (A) to (C) show the SD, statistics were computed with the non-parametric Mann-Whitney test with *P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001 and Tukey outliers. (D, F) display locomotion features of wt, the csb-1 mutant and the csb-1(ok2335);p_csb-1CSB-1::GFP rescue line 24 h after UVB irradiation, with (D) mean speed, (E) mean path curvature, and (F) mean path range (in A.U.) (n ≥ 60). Significance is measured via the Mann-Whitney test with *P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001, and Tukey outliers are shown as dots. Statistical significance in (A) to (F) without specific indication always refers to the colour-matched untreated control. (G) Experiment scheme of the chemotaxis assay (see Materials and Methods). (H) and (I) are chemotaxis assays in the wt, the csb-1 mutant and the csb-1(ok2335);p_csb-1CSB-1::GFP rescue line, quantified after 30 and 90 min, while (H) uses benzaldehyde, and (I) OP50 E. coli as attractant, respectively. Significance between zone-specific localization compared to the wt is measured by using the Welch's t-test with *P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001.