Fig. 3.

High proportion of Anergic B cells in the BM is significantly associated with negative survival outcome at 5 years independent of BM involvement. Proportions of anergic B cells in the BM of DLBCL patients at diagnosis were quantified using multicolor flow cytometry. Survival outcome at 5-years (yes/no) data was available on N = 24 BM samples but presence of clonal cells was detected in N = 9 of BM cases which were excluded in this analysis. The presence of these cells was determined and recorded previously as cells having an abnormal kappa: lambda light chain ratio with normal expression defined as 3:2 on flow cytometry. Comparison was made using exact non-parametric Mann-Whitney U test. For this analysis, p < .05 was taken to be significant. Graph represents mean ± SD