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. 2020 Oct 22;11:522059. doi: 10.3389/fpls.2020.522059

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Copyright © 2020 Dusek, Plchova, Cerovska, Poborilova, Navratil, Kratochvilova, Gunter, Jacobs, Hitzeroth, Rybicki and Moravec.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Schematic representation of the design of extended GoldenBraid assembly framework. (A) In the extended assembly the original α1 plasmid is replaced with 4 novel α-level plasmids in such a way that the 5′ end of α11 and 3′ end of α14 are identical to the ends of original α1. α2 and both Ω 1 and 2 plasmids are retained from GoldenBraid 2.0 system. (B) Examples of infinite loops in classical binary GoldenBraid assembly vs. extended assembly with the maximum number of assembled transcription units (Sarrion-Perdigones et al., 2013).