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Percentage of correctly assembled clones using either binary or 5-part assembly process.
| Assembly method | Total | Correct clones identified | Failure |
| Binary | 35 | 24 (68.6%) | 4 (9.5%) B; 7 (16.7%) C; 11 (31.4%) total |
| Quintuple | 144 | 115 (79.9%) | 12 (7.4%) A; 11 (6.7%) B; 6 (3.7%) C; 29 (20.1%) total |
A, competent cells failure, only colonies with no plasmid DNA; B, insufficient DTT levels in the reaction mixture, wrong and random restriction patterns; C, other, unknown.
