Fig. 7.
A-D) Summary of the contractile properties of popliteal lymphatics from induced and non-induced SMMHC-CreERT2;Kir6.1[GD-QR] mice. Expression of the GOF channel selectively in LMCs resulted in profound blunting of contraction amplitude at almost all pressures, ~no contractions at pressures ≤2 cmH2O and contraction frequencies ~1/2 of normal at other pressures. Calculated fractional pump flows in GOF vessels were 60-90% lower than in non-induced vessels at most pressures. Surprisingly, there were no significant differences in tone between the two groups. E-H) The application of 10 μM GLIB: reduced amplitude slightly at all pressures in vessels from non-induced mice (compare open symbols in A and E), but improved amplitude at all pressures (compare solid points in A and E); substantially increased frequency at all pressures in both induced and non-induced vessels; almost normalized fractional pump flow in the induced vessels; increased tone in the induced vessels by ~3-fold at all pressures. Means ± SD, n= 8 induced; n = 7 not-induced; * p < 0.05 between induced and non-induced; ǂ p < 0.05 between non-induced with vs without GLIB; # p < 0.05 between induced with vs without GLIB.