Table 4 -.

Pros and Cons of Different Experimental Approaches

	Transurethral	Transvesical
Pros	• Less damage to bladder wall • No requirement of abdominal surgery	• Can be performed on both females and males • Avoidance of influence on bladder neck/urethral closure • Feasibility of chronic catheter implantation • No necessity of anesthetic use during experiment
Cons	• Only possible to perform in females • Possible influence on bladder neck/urethral closure and resistance to flow • Requires anesthesia during experiment	• Damage to bladder wall • Requirement of abdominal surgery • Potential restriction of bladder contraction in longitudinal direction
	Single Fill	Continuous Fill
Pros	• Feasibility of analysis with greater accuracy of a variety of cystometry variables (e.g. true bladder capacity, post-void residual volume, and filling compliance)	• Convenient for quickly screening drug effects
Cons	• Unsuitability for screening short-acting drugs	• Inability to measure post-void residual volumes • Inability to measure filling parameters from a known empty bladder (e.g. true bladder capacity, filling compliance and baseline pressures)