Fig 1. Adult fat ablated mice are osteosclerotic.

(A-F) 2 month old control (Con) or DTR^ADQ mice were daily injected with Diphtheria Toxin (DT) (100ng/mouse/day) or PBS.

(A) Representative radiographs of femurs after 10 days of DT or PBS injection. n = 6/group.

(B) Representative μCT images of DTR^ADQ femurs, extending from metaphysis to diaphysis, with time of DT injection. n = 6/group.

(C) Quantitative μCT analysis of whole femur of Con or DTR^ADQ mice after 10 days of DT or PBS injection. n = 4–9/group.

(D) Representative histological section of femur of 2 month old DTR^ADQ mice treated with DT with time. n = 5/group. Control (Con) received PBS for 10 days; Scale bar: 800 μm.

(E) Representative μCT images of osmium-stained femurs of Con and DTR^ADQ mice 4 days after DT injection. n = 5/group. Marrow fat in dark gray (arrows), decalcified bone overlaid in light grey.

(F) Representative μCT images of vertebrae (L3–5) of Con and DTR^ADQ mice 10 days after DT injection. n = 5/group.

(G) 2 month old Con or DTR^ADQ mice were subjected to ovariectomy or sham operation. 3 weeks after surgery, mice were daily injected with DT for 10 days. Whole femur BV/TV and BMD were analyzed by μCT. n = 2–6/group.

(H) 2 month or 1 year old Con or DTR^ADQ mice were daily injected with DT for 10 days. Whole femur was analyzed by μCT. n = 3–7/group.

Data are presented as mean ± SD. * P < 0.05, *** P < 0.001 as determined by 2 way ANOVA with Holm-Sidak’s post hoc analysis for multiple comparisons test (C, G, H).