| Antibodies |
| Rabbit anti-phospho-smad |
Cell Signaling Biotechnology |
Cat# 13820; RRID: AB_2493181 |
| Rabbit anti-Smad5 |
Cell Signaling Biotechnology |
Cat# 12534; RRID: AB_2797946 |
| Rabbit anti-Adiponectin |
Cell Signaling Biotechnology |
Cat# 2789; RRID: AB_2221630 |
| Rabbit anti-phospho EGFR |
Cell Signaling Biotechnology |
Cat# 3777; RRID: AB_2096270 |
| Rabbit anti-EGFR |
Cell Signaling Biotechnology |
Cat# 2232; RRID: AB_331707 |
| Mouse anti-PCNA |
Santa Cruz Biotechnology |
Cat# SC-56; RRID: AB_628110 |
| Mouse anti-Actin |
Sigma Aldrich |
Cat# A2228; RRID: AB_ 476697 |
| Rabbit anti-Thymidine kinase |
William Summers (Yale) |
N/A |
| Rat anti-mouse B220-PE |
BioLegend |
Clone RA3-6B2; RRID: AB_312993 |
| Rat anti-mouse CD45-APC |
BioLegend |
Clone 30-F11 ; RRID: AB_312976 |
| Rat anti-mouse CD19 APC/Fire750 |
BioLegend |
Clone 6D5; RRID: AB_2572120 |
| FcBlock |
BD Biosciences |
Clone 24G.2; RRID: AB_394656 |
| Chemicals, Recombinant Proteins |
| Diphtheria Toxin |
Biological List Laboratories |
Cat #150 |
| Tamoxifen |
Sigma Aldrich |
Cat# T5648 |
| LDN193189 |
Sigma Aldrich |
Cat# SML0559 |
| XAV939 |
Selleckchem |
Cat# S1180 |
| Gefitinib |
ApeBio |
Cat# A8219 |
| Ganciclovir |
Acros Organics |
Cat# 461710050 |
| TRIzol Reagent |
ThermoFisher Scientific |
Cat# 15596026 |
| Osmium Tetroxide 4% solution |
Electron Microscopy Sciences |
Cat# 19170 |
| Sorensen’s Phosphate buffer |
Electron Microscopy Sciences |
Cat# 11600-10 |
| Collagenase P |
Roche |
Cat# 11249002001 |
| Ascorbic Acid |
Sigma Aldrich |
Cat# A5960 |
| β-glycerophosphate |
Sigma Aldrich |
Cat# G9422 |
| Indomethacin |
Sigma Aldrich |
Cat# I7378 |
| Dexamethasone |
Sigma Aldrich |
Cat# D4902 |
| Insulin |
Sigma Aldrich |
Cat# I2643 |
| OPG-Fc |
Amgen Biotechnology |
N/A |
| Recombinant Human HB-EGF |
R&D Systems |
Cat# 259-HE-050 |
| MEM Alpha |
Gibco |
Cat# 12561-049 |
| FBS |
Gibco |
Cat# 26140 |
| 0.05% Trypsin-EDTA |
Gibco |
Cat# 25300-054 |
| Pen Strep |
Gibco |
Cat# 15140-122 |
| 30% Acrylamide/Bis Solution |
Bio-Rad |
Cat# 161-0158 |
| Halttm Protease & Phosphatase inhibitor |
Thermo Scientific |
Cat# 78443 |
| 50% Dextrose |
Hospira |
Cat# 0409-6648-02 |
| Oligonucleotides |
| See Supplemental Table 3, 4
|
|
|
| Critical Commercial Assays |
| iScript™ cDNA synthesis kit |
Bio-Rad |
Cat# 170-8891 |
| Powerup™ SYBR™ Green |
Applied Biosystems |
Cat# 25742 |
| Serum CTX-1 (RatLaps) RIA kit |
Immunodiagnosticsystems |
Cat# AC—06F1 |
| Mouse P1NP EIA kit |
Immunodiagnosticsystems |
Cat# AC-33F1 |
| RNeasy Mini kit |
Qiagen |
Cat# 74104 |
| LDH activity Assay kit |
BioVision Incorporated |
Cat# K726-500 |
| Deposited Data |
| RNA sequencing data |
GEO |
GEO: GSE154800
|
| Experimental Models: Organisms/Strains |
| iDTR (Lox-Stop-Lox-ROSA-DTR) |
The Jackson Laboratory |
Stock No: 007900 |
| Col1a1*2.3GFP |
The Jackson Laboratory |
Stock No: 013134 |
| CD19 Cre |
The Jackson Laboratory |
Stock No: 006785 |
| Adipoq Cre |
The Jackson Laboratory |
Stock No: 010803 |
| Adipoq Cre/ERT2 |
The Jackson Laboratory |
Stock No: 025124 |
| TK-3.6Col1a1 |
Robert Jilka and Charles O’Brien (University of Arkansas) (Jilka et al., 2009) |
N/A |
| Rosa-DTA (Lox-Stop-Lox-ROSA-DTA) |
The Jackson Laboratory |
Stock No: 010527 |
| Experimental Models: Cell Line |
| ST2 |
ATCC |
CVCL-2205 |
| Software and Algorithms |
| GraphPad Prism 8 |
GraphPad |
RRID:SCR_002798 |
| Bioquant Osteo V7 10.10 |
Bioquant |
RRID:SCR_016423 |
| Image Studio Lite Ver4.0 |
LI-COR Biosciences |
RRID:SCR_013715 |
| STAR v2.0.4b |
STAR |
RRID:SCR_015899 |
| Subread v1.4.5 |
Subread |
RRID:SCR_009803 |
| R v3.4.1 |
R |
RRID:SCR_001905 |
| EdgeR v3.20.2 |
Edger |
RRID:SCR_012802 |
| LIMMA v3.34.4 |
LIMMA |
RRID:SCR_010943 |
| RSeQC v2.3 |
RSeQC |
RRID:SCR_005275 |
| CompBio |
http://www.percayai.com |
N/A |
| Other |
| Immobilon-FL PVDF transfer membranes |
Millipore |
Cat# IPFL00010 |
| Contour Blood Glucose Strips |
ASCENSIA Diabetes Care |
Cat# 7097C |
| Contour Blood Glucose Meter |
Bayer Health LLC |
Cat# 9556C |
| BD Serum Separator Tubes |
Becton, Dickinson and Company |
Cat# 365967 |
| Plain Capillary Tubes |
Kimble Chase Life Science |
Cat# 2502 |
