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. 2020 Oct 22;8:553728. doi: 10.3389/fcell.2020.553728

FIGURE 6.

FIGURE 6

Overexpressed Six3os1 greatly alleviates oxidative stress of neurons in mice with CUMS-induced depression in vivo. CUMS-induced mice were injected with adenovirus of oe-NC + sh-NC, oe-Six3os1 + sh-NC and oe-Six3os1 + sh-Fezf1, and CUMS-induced mice administered with GP100 were injected with sh-NC + oe-NC, sh-Six3os1 + oe-NC and sh-Six3os1 + oe-Fezf1. (A) The injection of adenovirus and green fluorescence expression. (B) Six3os1 and Fezf1 expression in mice detected by RT-qPCR. (C,D) Immunoblots (C) and quantitation (D) of Fezf1 protein expression normalized to GAPDH in mice examined by Western blot analysis. (E) Sucrose preference (%) of mice after different treatments. (F) Retention time in open field (s) of mice after different treatments. (G) Memory ability of mice after different treatments evaluated by Morris water maze. (H) Tail suspension time (s) of mice after different treatments. (I) Forced swimming time (s) of mice after different treatments. (J) The weight of mice at day 42. (K) SOD, GSH, CAT, and MAD contents in mice determined by ELISA. The measurement data were presented as mean ± standard deviation. Data between two groups were compared by independent sample t-test, and data among multiple groups were analyzed by one-way ANOVA, followed by Tukey’s post hoc test. *p < 0.05 vs. CUMS-induced mice treated with oe-NC + sh-NC and the CUMS-induced mice administered with GP after treatment of sh-NC + oe-NC; #p < 0.05 vs. the CUMS-induced mice treated with oe-Six3os1 + sh-NC and CUMS-induced mice administered with GP after treatment of sh-Six3os1 + oe-NC.