TABLE 1.
Gene | Primer sequence (5′-3′) |
Six3os1 | F: TGTAGCAGCGGCGGATAAAT |
R: AGGCTTCCCAACAGGTTAGC | |
Fezf1 | F: ACGCGACCACCAAAATGCTA |
R: TCGACGAGTTGAGATGCAGAG | |
GAPDH | F: AGGTCGGTGTGAACGGATTTG |
R: TGTAGACCATGTAGTTGAGGTCA | |
miR-511-3p | F: GCAGAATGTGTAGCAAAAGACA |
R: GGTCCAGTTTTTTTTTTTTTTTATCCT | |
U6 | F: GCTTCGGCAGCACATATACTAAAAT |
R: CGCTTCACGAATTTGCGTGTCAT |
RT-qPCR, reverse transcription quantitative polymerase chain reaction; F, forward; R, reverse; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; miR-511-3p, microRNA-511-3p.