Fig. 4.

Overexpression of wild-type TUBB8 cRNA improves impaired spindle assembly. a–c Immunostaining, bipolar spindle formation rates, and spindle length in mouse oocytes at 14 h after germinal vesicle breakdown (GVBD). GV oocytes were injected with mutant or co-injected with mutant and wild-type TUBB8 cRNA. d–f Immunostaining, bipolar spindle formation rates, and spindle length of mouse zygotes at 14 h after fertilization; analysis followed injection with mutant or co-injection with mutant and wild-type TUBB8 cRNA. The data are represented as the mean ± SEM, *p < 0.05, **P < 0.01. Scale bar = 5 μm