Influence of environmental Fe on B. subtilis growth and Fe acquisition strategy. Panels show cellular growth (A), total intracellular Fe concentration (B), isotopic composition of biofilm-bound Fe (C), and isotopic distribution of new cellular Fe (D) of B. subtilis cells 3 h and 6 h after transfer in a Fe-depleted MSgg medium (white bars in panels B, C, and D, and triangles in panel A), 10−4 M 57FeCl3 (dark gray bars in panels B, C, and D, and circles in panel A), or 10−4 M 57Fe-tannic acid (light gray bars in panels B and D, and squares in panel A).