TABLE 4.

S. thermophilus N4L genes significantly differentially expressed during growth in two yeast extracts

Locus	Annotation	Pathway	FCa (Y2/Y1)			log2 FC
			t3	t4	t5	t3	t4	t5
STN4L_00055	Amino acid ABC transporter (substrate binding protein)	Amino acid metabolism	1.69	0.46	0.58	0.76	−1.11	−0.78
STN4L_00056	Acetate kinase	Purine metabolism	1.41	0.42	0.40	0.50	−1.25	−1.32
STN4L_00092	Protein of unknown function		0.70	0.57	0.47	−0.52	−0.82	−1.09
STN4L_00094	Oxalate:formate antiporter		2.29	1.09	1.12	1.19	0.12	0.16
STN4L_00157	Protein of unknown function	Streptide metabolism	1.35	0.11	0.02	0.44	−3.20	−5.97
STN4L_00158	KXXXW cyclic peptide radical SAM maturase	Streptide metabolism	1.24	0.33	0.16	0.31	−1.61	−2.66
STN4L_00159	Uncharacterized protein	Streptide metabolism	1.43	0.37	0.23	0.52	−1.45	−2.15
STN4L_00160	Coenzyme PQQ synthesis protein	Coenzyme metabolism	1.43	0.29	0.18	0.52	−1.79	−2.47
STN4L_00162	Uncharacterized protein		1.83	0.41	0.24	0.87	−1.28	−2.05
STN4L_00163	Protein of unknown function		1.15	0.38	0.19	0.20	−1.40	−2.42
STN4L_00193	Uncharacterized protein		2.84	1.92	1.16	1.51	0.94	0.21
STN4L_00208	Protein of unknown function		0.68	0.64	2.03	−0.55	−0.63	1.02
STN4L_00212	Hypothetical protein		0.77	0.40	0.20	−0.38	−1.31	−2.31
STN4L_00220	Uncharacterized protein		1.07	1.56	2.20	0.10	0.64	1.13
STN4L_00223	Uncharacterized protein		1.43	2.04	1.99	0.51	1.03	0.99
STN4L_00290	Aconitate hydratase	Citrate metabolism	0.26	0.66	0.74	−1.93	−0.60	−0.43
STN4L_00291	Citrate synthase	Citrate metabolism	0.27	0.68	0.87	−1.89	−0.55	−0.20
STN4L_00292	Isocitrate dehydrogenase (NADP)	Citrate metabolism	0.28	0.70	0.81	−1.85	−0.52	−0.30
STN4L_00335	Histidinol-phosphate aminotransferase	Amino acid metabolism (His)	1.19	1.66	2.10	0.25	0.73	1.07
STN4L_00337	ATP phosphoribosyltransferase	Amino acid metabolism (His)	1.25	1.84	2.16	0.32	0.88	1.11
STN4L_00340	Imidazole glycerol phosphate synthase subunit HisH	Amino acid metabolism (His)	1.19	1.64	2.00	0.25	0.71	1.00
STN4L_00439	Uncharacterized protein		2.14	1.32	1.18	1.10	0.40	0.24
STN4L_00612	Protein of unknown function		0.53	0.67	0.42	−0.92	−0.57	−1.25
STN4L_00652	Acetoin dehydrogenase	Acetoin metabolism	0.82	0.47	1.07	−0.29	−1.09	0.10
STN4L_00714	Uncharacterized protein		0.21	0.48	2.80	−2.26	−1.06	1.48
STN4L_00715	Protein of unknown function		0.39	0.47	0.59	−1.37	−1.08	−0.77
STN4L_00866	Protein of unknown function		0.34	0.80	0.70	−1.57	−0.32	−0.51
STN4L_00906	Hypothetical protein		0.81	1.27	2.15	−0.30	0.35	1.11
STN4L_00969	Ornithine carbamoyltransferase	Amino acid metabolism (Arg)	1.55	0.47	0.54	0.63	−1.08	−0.88
STN4L_01004	Uncharacterized protein		1.42	0.94	2.12	0.51	−0.10	1.09
STN4L_01063	Carbamoyl-phosphate synthase (glutamine hydrolyzing)	Pyrimidine metabolism	0.37	0.91	0.84	−1.45	−0.14	−0.25
STN4L_01064	Carbamoyl-phosphate synthase small chain	Pyrimidine metabolism	0.48	0.96	0.77	−1.05	−0.06	−0.38
STN4L_01126	Uncharacterized protein		1.72	1.93	2.28	0.78	0.95	1.19
STN4L_01129	Arginine biosynthesis bifunctional protein ArgJ	Amino acid metabolism (Arg)	1.62	0.51	0.49	0.70	−0.96	−1.03
STN4L_01175	Transcriptional regulator	Regulator	0.42	0.82	0.95	−1.27	−0.28	−0.08
STN4L_01277	Hypothetical protein		4.84	2.79	1.73	2.27	1.48	0.79
STN4L_01422	Uncharacterized protein		2.18	1.55	1.42	1.12	0.64	0.51
STN4L_01581	ISSth1, transposase (Orf2), IS3 family		1.16	2.05	2.19	0.21	1.03	1.13
STN4L_01647	Bifunctional purine biosynthesis protein	Purine metabolism	2.11	0.99	0.97	1.08	−0.01	−0.05
STN4L_01648	Phosphoribosylglycinamide (GAR) formyltransferase	Purine metabolism	2.27	0.97	0.91	1.18	−0.05	−0.13
STN4L_01649	Phosphoribosylformylglycinamidine cyclo-ligase	Purine metabolism	2.55	0.98	0.87	1.35	−0.03	−0.21
STN4L_01650	Amidophosphoribosyltransferase	Purine metabolism	2.50	1.08	0.99	1.32	0.12	−0.01
STN4L_01866	A0A249DMB6 (uncharacterized protein)		2.02	1.05	0.71	1.01	0.07	−0.50
STN4L_01963	Argininosuccinate synthase	Amino acid metabolism (Arg)	1.38	0.41	0.52	0.46	−1.29	−0.93
STN4L_01964	Argininosuccinate lyase	Amino acid metabolism (Arg)	1.80	0.48	0.45	0.85	−1.06	−1.17
STN4L_01998	Transposase (IS1193)		1.08	2.30	1.57	0.11	1.20	0.65
STN4L_02026	Uncharacterized protein		0.59	0.42	0.29	−0.76	−1.27	−1.78
STN4L_02035	Protein of unknown function		0.63	0.64	3.23	−0.67	−0.65	1.69
STN4L_02079	Response regulator of the LytR/AlgR family protein	Regulator	0.48	0.93	0.88	−1.07	−0.11	−0.18
STN4L_02180	GTP-sensing transcriptional pleiotropic repressor CodY	Regulator	0.77	1.17	2.07	−0.38	0.22	1.05
STN4L_02182	Lipoprotein		1.57	1.15	2.36	0.65	0.20	1.24
STN4L_02206	Uncharacterized protein		0.40	0.86	1.60	−1.33	−0.22	0.67
STN4L_02239	Amino acid ABC transporter permease	Amino acid metabolism (Gln)	2.87	0.41	0.55	1.52	−1.30	−0.87
STN4L_02240	Amino acid ABC transporter permease	Amino acid metabolism (Gln)	3.45	0.42	0.59	1.79	−1.25	−0.75
STN4L_02241	Glutamine ABC transporter, ATP-binding protein GlnQ	Amino acid metabolism (Gln)	3.47	0.45	0.78	1.80	−1.15	−0.36
STN4L_02242	Amino acid ABC transporter amino acid-binding protein	Amino acid metabolism (Gln)	2.56	0.44	0.56	1.36	−1.19	−0.84
STN4L_02252	Uncharacterized protein		0.89	0.24	0.04	−0.17	−2.06	−4.64
STN4L_02254	Uncharacterized protein		1.23	0.24	0.05	0.30	−2.04	−4.47

^aFC, fold change.