TABLE 3.
Mutations identified in phage-resistant 10403S mutants selected by LP-048, LP-125, or both
| Nucleotide position | Gene homolog |
Description | Mutation typea | WT | Altb | Strain | No. of mutations | Phage selection | Susceptibility to: |
||
|---|---|---|---|---|---|---|---|---|---|---|---|
| 10403S | EGD-e | LP-048 | LP-125 | ||||||||
| 1097413 | LMRG_00542 | lmo1080 | Rhamnosyltransferase (RmlT) | NS | C | T | UTK P1-0003 | 3 | LP-048 | − | − |
| 1098886 | LMRG_00544 | lmo1082 | dTDP-4-dehydro-rhamnose 3,5-epimerase (RmlC) | FS | GAATA | G | UTK P1-0001 | 1 | LP-048 | − | + |
| 1098886 | LMRG_00544 | lmo1082 | dTDP-4-dehydro-rhamnose 3,5-epimerase (RmlC) | FS | GAATA | G | UTK P1-0007 | 3 | LP-048 | + | − |
| 1099454 | LMRG_00545 | lmo1083 | dTDP-glucose 4,6-dehydratase (RmlB) | MS | C | T | UTK P1-0006 | 1 | LP-048 | − | + |
| 1099515 | LMRG_00545 | lmo1083 | dTDP-glucose 4,6-dehydratase (RmlB) | MS | A | G | UTK P1-0004 | 2 | LP-048 | − | − |
| 1099619 | LMRG_00545 | lmo1083 | dTDP-glucose 4,6-dehydratase (RmlB) | MS | G | A | UTK P1-0005 | 1 | LP-048 | − | + |
| 1099958 | LMRG_00546 | lmo1084 | dTDP-4-dehydro-rhamnose reductase (RmlD) | NS | T | G | UTK P1-0002 | 1 | LP-048 | − | + |
| 1235730 | LMRG_00695 | lmo1249 | Hypothetical protein | Intergenic | T | TA | UTK P1-0007 | 3 | LP-048 | + | − |
| 1493524 | LMRG_01466 | lmo1504 | Alanyl tRNA synthetase (AlaS) | MS | C | T | UTK P1-0003 | 3 | LP-048 | − | − |
| 1770042 | LMRG_02528 | lmo1743 | Hypothetical protein | FS | CT | C | UTK P1-0007 | 3 | LP-048 | + | − |
| 1890882 | LMRG_02979 | lmo1859 | Peptide-methionine (R)-S-oxide reductase (MsrB) | MS | C | T | UTK P1-0004 | 2 | LP-048 | − | − |
| 2573008 | LMRG_01710 | lmo2537 | UDP-N-acetyl-glucosamine 2-epimerase | Intergenic | C | T | UTK P1-0003 | 3 | LP-048 | − | − |
a
NS, nonsense; MS, missense; FS, frameshift; the first nucleotide is maintained from WT to Alt in frameshift mutations.
b
Alt, alternate allele.