TABLE 1.
qPCR primers used to quantify host and viral copy numbers during the 2019 viral lysis event
| Target organism | Gene | Forward primer | Reverse primer | Source |
|---|---|---|---|---|
| Microcystis aeruginosa | 16S | GCCGCRAGGTGAAAMCTAA | AATCCAAARACCTTCCTCCC | (50) |
| Microcystis aeruginosa | mcyB | CCTACCGAGCGCTTGGG | GAAAATCCCCTAAAGAATCCTGATTCCTGAGT | (51) |
| Microcystis aeruginosa | mcyD | GGTTCGCCTGGTCAAAGTAA | CCTCGCTAAAGAAGGGTTGA | (36) |
| Ma-LMM01 | gp091 | ACATCAGCGTTCGTTTCGG | CAATCTGGTTAGGTAGGTCG | (14) |
| MVGF_J_19 | Phage collar | GCGGGCGTTTGCATTTATT | ATCTGCTGTAACTCACCATCAC | This study |
| MVGF_J_348 | Phage portal | CACGACTGAATCACCCGATAG | GGGCTATCCTATGCGGCTTTATC | This study |