TABLE 2.

Fe(II) and total Fe concentrations in the growth medium before and after AMB-1 cultures and final optical density and pH values of bacterial cultures

Time of culture (h) by concn	Vol of culture (ml)	Initial [Fe(II)] (μM)	Initial [Fe] (μM)	Final OD400 (AUa)	Final pH	Final [Fe(II)] (μM)	Final [Fe] (μM)
[Fe] = 30 μM
26	185	16.87	31.36	0.067	6.95	7.29	30.94
	185	17.24	31.72	0.060	6.91	11.03	30.94
	185	17.05	31.72	0.083	7.01	28.45	30.94
46	185	14.12	32.46	0.209	7.26	24.99	24.30
	185	13.94	32.82	0.202	7.34	24.99	26.03
	185	14.85	31.72	0.214	7.26	24.12	24.12
69	185	8.80	33.19	0.212	7.42	28.12	27.42
	185	8.44	33.19	0.203	7.44	28.47	27.07
	185	7.52	33.74	0.204	7.42	26.20	26.20
[Fe] = 150 μM
26	185	29.16	162.65	0.069	7.03	51.57	154.89
	185	26.96	157.15	0.067	6.94	35.21	152.40
	185	26.41	159.35	0.056	6.94	25.96	154.18
46	185	26.77	161.00	0.215	7.18	57.80	140.24
	185	23.29	158.25	0.215	7.28	54.15	138.85
	185	20.90	161.73	0.211	7.30	56.58	136.94
69	185	15.95	160.27	0.197	7.48	143.39	146.88
	175	15.95	172.00	0.202	7.50	154.57	162.08
	185	14.12	158.80	0.202	7.50	143.74	141.99

^aAU, absorbance unit.