TABLE 3.
Fe(II) and total Fe concentrations in the growth medium before and after mutant AMB-1 cultures, and final optical density and pH values of bacterial culturesa
| Time of culture (h) by strain | Vol of culture (ml) | Initial [Fe(II)] (μM) | Initial [Fe] (μM) | Final OD400 (AU) | Final pH | Final [Fe(II)] (μM) | Final [Fe] (μM) |
|---|---|---|---|---|---|---|---|
| Wild type | |||||||
| 43 | 178 | 25.08 | 150.30 | 0.250 | 7.30 | 26.23 | 145.78 |
| 173 | 22.85 | 155.69 | 0.252 | 7.24 | 25.23 | 150.59 | |
| 167 | 23.22 | 164.05 | 0.255 | 7.40 | 19.42 | 151.99 | |
| ΔmamP | |||||||
| 43 | 171 | 21.55 | 159.59 | 0.261 | 7.32 | 21.43 | 158.20 |
| 174 | 19.32 | 161.27 | 0.252 | 7.35 | 18.23 | 161.00 | |
| 170 | 18.21 | 162.75 | 0.261 | 7.33 | 14.42 | 159.20 | |
| ΔmamT | |||||||
| 43 | 175 | 16.72 | 157.92 | 0.248 | 7.34 | 15.02 | 158.20 |
| 177 | 15.42 | 153.23 | 0.280 | 7.35 | 15.62 | 148.29 | |
| 164 | 14.31 | 169.44 | 0.252 | 7.39 | 10.21 | 172.22 |
a
Additional wild-type cultures were used as a control condition.