TABLE 2.
Primers used in this study
| Purpose | Primer | Sequence (5' to 3') |
|---|---|---|
| qRT-PCR analysis | 16S_qF | GCGCAGAACCTTACCAACGT |
| 16S_qR | AGCCATGCAGCACCTGTCA | |
| desA_qF | GCCTTCGCCTTCCTCAACTA | |
| desA_qR | CACCGGAACCCACTGCTT | |
| ligM_qF | GCTCTCCGACACGATGATCA | |
| ligM_qR | ACGTACTGCTTCGCCTTGTTG | |
| desB_qF | TTTCGAGCATTATTCGCATTTC | |
| desB_qR | TCCGCAGGCGAATATTCCT | |
| ferB_qF | CCGGTGGAACGGGAAGA | |
| ferB_qR | CCACGCCACGTTGTTCAC | |
| 25010_qF | GACATGCTGTGGCAGATGTG | |
| 25010_qR | CGCATCTGCCGCTCATAC | |
| desX_qF | CAGAAGGTGGACTCGTCGT | |
| desX_qR | AGGATATCGAGCGTGCG | |
| RT-PCR analysis | RTferB_F | TGACGTACGACAATGCGGAA |
| RTferA_F | TGACGCCTCCATCATTCTCG | |
| RTferA_R | ATGATCCGCGTCTTCTCGTC | |
| RT25010_F1 | ATGAGTCACTCGCCTTCCA | |
| RT25010_F2 | TCAGCACCGGCATTCACTT | |
| RT25010_R | GCATCGATGAGGGCATCCAT | |
| RTdesA_R1 | CCGACGAGGTTGAACTGGTT | |
| RTdesA_R2 | TCATAATCCGCCCAGGGAC | |
| Gene complementation | pJB24970_F | GACGTCACCATGGGAAGCTTGACACGATCTACCTGCGCA |
| pJB24970_R | CCTGCAGGATATCTGGATCCTCCTCGTGGGACTGGTCAT | |
| Primer extension | PE25010a | ACGGTCATCGCAGATCAG |
| Promoter assay | pSDA1_F | ACCTGCAGGCATGCAAGCTTTCCATCATTCTCGACGGCG |
| pSDA1_R | ATGTTTTTCCTCCTAAGCTTTCAGTCCACCAGCATCAGG | |
| pSDA2_F | ACCTGCAGGCATGCAAGCTTGCGCATCCAGGAACTCGAT | |
| pSDA2a_F | ACCTGCAGGCATGCAAGCTTGCATGACCTTTCAATTGTGCG | |
| pSDA2b_F | ACCTGCAGGCATGCAAGCTTCGTATATACGAAGAACATCGG | |
| pSDA2c_F | ACCTGCAGGCATGCAAGCTTCGGATTTCCATGAGTCACTCG | |
| pSDA2_R | ATGTTTTTCCTCCTAAGCTTTTCCCCATAGCCCGCAA | |
| pSDA3_F | ACCTGCAGGCATGCAAGCTTCCTGATCTGCGATGACCGT | |
| pSDA3_R | ATGTTTTTCCTCCTAAGCTTCGCATCTGCCGCTCATAC | |
| pSDA4_F | ACCTGCAGGCATGCAAGCTTGACATGCTGTGGCAGATGTG | |
| pSDA4_R | ATGTTTTTCCTCCTAAGCTTTTCCGGCATTGTCCAGCA | |
| Protein expression | p16bdX_F | TATCGAAGGTCGTCATATGATCCAGAAGGTGGACTC |
| p16bdX_R | CTTTGTTAGCAGCCGGATCCTCAGCGGTCGCCAAG | |
| EMSAs | desAp1_F | CTGCAGGATGTGCGCC |
| desAp1_R | GTGAATGCCAGCCCCAAA | |
| desAp2_F | GCATGACCTTTCAATTGTGCG | |
| desAp2_R | AAGTGAATGCCGGTGCTGAT | |
| desAp3_F | AGATCATTCGCCGCGCA | |
| desAp3_R | ATGGCTTCATGCTGCACC | |
| desAp4_F | ACCGGCGCCGATGATGC | |
| desAp4_R | TGGTTGAAGAGCACGGCGG | |
| desAp5_F | AACTGGCGCAACGAGCA | |
| desAp5_R | CTGCAGCTGGAAGCGATAGT | |
| desAp6_F | ATGAGTCACTCGCCTTCCA | |
| desAp7_F | TTTCCCTCTGCACGACGT | |
| desAp7_R | GTGCCCAGATACGGTCATC | |
| desAp8_F | GCGCATCCAGGAACTCGAT | |
| desAp8_R | TTTCATCTACTCGAAGACCGG | |
| IR-DA_R | AATCCGATGTTCTTCGTATATACGAAGATTTCATCT | |
| IR-DA_mL_R | AATCCGATGTTCTTCGTATGCGTAGGAGTTTCATCT | |
| IR-DA_mR_R | AATCCGATGTCTCCTACGCATACGAAGATTTCATCT | |
| ferBp_F | ATATTGTCGAGCGGGCTG | |
| ferBp_R | ATCTGGCGGTTGAGCTTG | |
| desBp_F | GCCGTTCCCCTCTCAGGC | |
| desBp_R | AAACCGCCGATGATCTTTGC | |
| ligMp_F | GTTTCCGCCTGGTCTGAA | |
| ligMp_R | AGGTGCCGACATCAGCTA |
a
Primer labeled with 6-carboxyfluorescein at the 5′ end.