Key Resources Table
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Anti-6X HIS Tag PE | AbCam | Cat# ab72467 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Human CD160-His | R&D Systems | Cat# 6177-CD-050 |
| Human BTLA-His | R&D Systems | Cat# 9235-BT-050 |
| Human LIGHT (TNF14) His | R&D Systems | Cat# 664-LI-025 |
| Deposited Data | ||
| Human HVEM and BTLA complex | (Compaan, et al., 2005) | PDB: 2AW2 |
| Human HVEM and LIGHT complex | N/A | PDB: 4RSU |
| Human HVEM and CD160 complex | (Liu, et al., 2019) | PDB: 6NG3 |
| Experimental Models: Cell Lines | ||
| HEK 293-F Freestyle | Thermofisher | CAt# R79007 |
| Oligonucleotides | ||
| DNA primers | see Table S3 for all primers | N/A |
| Recombinant DNA | ||
| human HVEM (TNFRSF14) | GeneCopoeia | Cat# EX-M0900-M98 |
| Software and Algorithms | ||
| Modeller | (Sali and Blundell, 1993) | https://salilab.org/modeller/ |
| PyMOL | PyMOL version 1.7 | https://sourceforge.net/projects/pymol/files/pymol/1.7/ |
| Prism | Prism version 8.3.0 | https://www.graphpad.com/scientific-software/prism/ |
| INKSCAPE | INKSCAPE version 0.92 | https://inkscape.org |
| Python | Python version 3.7.5 | https://www.python.org |
| StatsModels | StatsModels version 0.10.2 | https://www.statsmodels.org/stable/index.html |
| pandas | pandas version 0.25.3 | https://pandas.pydata.org/index.html |
| NumPy | NumPy version 1.17.4 | http://www.numpy.org |
| SciPy | SciPy version 1.3.3 | https://docs.scipy.org/doc/scipy/reference/stats.html |
| Matplotlib | Matplotlib version 3.1.2 | https://matplotlib.org |
| ProtLID | (Yap and Fiser, 2016) | N/A |