Table 1.

Putative miR-92a target genes

Gene name	Flybase ID	RNAi line	PI for nSyb-GAL4 screen	Retest score	p value
CG12024	CG12024	GD-20143	0.45 ± 0.02
CG8360	CG8360	GD-41643	0.40 ± 0.08
cpr50Ca	CG13338	KK-100317	0.64 ± 0.07	0.56 ± 0.08	0.15
khc-73	CG8183	KK-105984	0.38 ± 0.09	0.28 ± 0.07	0.02*
crebA	CG7450	KK-110650	0.37 ± 0.06	0.37 ± 0.08	0.09

Candidate mRNA targets for miR-92a were selected by applying the pipeline described in Figure 5A. Five genes were screened for a role in memory formation using an RNAi approach. The five lines from the Vienna Drosophila RNAi left (https://stockcenter.vdrc.at/control/main) that were tested are listed. RNAi lines were crossed to the nSyb-GAL4 driver and tested for 3-h memory with n = 4. Each individual RNAi line was compared with a daily nSyb-GAL4>UAS-dcr-2 control in the respective GD or KK background. The average performance index for the nSyb-GAL4>UAS-dcr-2 control was 0.36 ± 0.1 for the GD control (n = 4) and 0.49 ± 0.04 for the KK control. Three lines with a trend for a significant effect on memory were retested with n = 6. Only the khc-73 RNAi line, had a PI significantly lower than the control (n = 10). Results shown are the mean ± SEM. Two-tailed, two-sample Student’s t tests, *p < 0.05.