Fig. 4.

Substrates that demonstrated significant mitochondrial utilization differences based on A) the number of mature oocytes retrieved and B) the percent mature oocytes retrieved from each patient. To determine the differences in mitochondrial utilization, substrates were compared using a multiple linear regression model using Box-Cox transformation. Individuals with a greater number of mature oocytes and percent mature oocytes retrieved were found to have a significant increase in alpha-keto-isocaproic acid mitochondrial utilization (P < 0.01) in pooled GCs of patients, after adjusting for the other factors in the model. Following the Box-Cox transformation of the data and observing the Q-Q plots, the most outlying observations have been removed to ensure the normality of the response variables. Data are presented as a scatter plot of the individual patient values lambda transformed for normalization showing the trend line and the 95% confidence interval