Extended Data Fig. 3. Gli1 Lin+ mesenchyme expands distally in fibrotic repair.

(a) Lack of Gli1 Lin+ cells in corn oil-treated Gli1^creERT2:R26^YFP lung (2 animals).

(b,c) Comparison of Gli1 Lin+ cell localization with Gli1+ cells in Gli1^LacZ/+ lung. These experiments were each repeated three times independently with similar results.

(d,e) Extended washout after tamoxifen (>4wks) followed by bleomycin demonstrates similar expansion of Gli1 Lin+ cells into the alveoli. (n = 3 per group; each data point represents one animal; one-tailed unpaired Student’s t-test). Data are expressed as mean ± SD.

(f,g) Deletion of Gli1+ cells does not alter airway epithelial composition during normal homeostasis. (n = 3 per group; each data point represents one animal; one-tailed unpaired Student’s t-test). Data are expressed as mean ± SD.

(h-j) Gli1-deleted lungs treated with bleomycin demonstrate reduced expression of Gli1 along with changes in fibrotic markers (n = 6 for control, n = 5 for Gli1-deleted; each data point represents one animal; for (i), Col1a1 P = 0.0862; Snai1 P = 0.3126; Cdh2 P = 0.0310; one-tailed unpaired Student’s t-test for (h-j)). Data are expressed as mean ± SD.

(k) Weight loss after bleomycin injury in control and Gli1-deleted animals (n = 6 for control, n = 5 for Gli1-deleted; each data point represents one animal; one-tailed unpaired Student’s t-test). Data are expressed as mean ± SD.

AW = airway. Scale bars, 100 µm.