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. Author manuscript; available in PMC: 2021 Apr 12.
Published in final edited form as: Nat Cell Biol. 2020 Oct 12;22(11):1295–1306. doi: 10.1038/s41556-020-00591-9

Extended Data Fig. 7. Spatial regulation of BMP activation.

Extended Data Fig. 7

(a,b) pSMAD1/5/8 staining of Sox2 Lin+ cells in the alveoli after bleomycin injury demonstrates that the majority of pSMAD1/5/8+/Sox2 Lin+ cells are SCGB1A1+ or SFTPC+, and rarely KRT5+ (n = 3 for KRT5 quantification, n = 4 for SCGB1a1 and SFTPC quantification; each data point represents one animal). Data are expressed as mean ± SD.

(c) qPCR of Hh-inducible cultured mesenchyme isolated from UbccreERT2/+:R26SmoM2/+ lungs treated with 4OHT +/- TGF-β, showing effect on various BMP antagonists expression (n = 3; each data point represents one biological replicate). Wnt5a: Control v TGF-β P = 0.0094, Control v Hh-activated P = 0.1066, Control v TGF-β + Hh-activated P = 0.002; Fst: Control v TGF-β P = 0.0598, Control v Hh-activated P = 0.0389, Control v TGF-β + Hh-activated P = 0.3833; Fstl1: Control v TGF-β P = 0.2051, Control v Hh-activated P = 0.4620, Control v TGF-β + Hh-activated P = 0.028; Fstl3: Control v TGF-β P = 0.0005, Control v Hh-activated P = 0.0704, Control v TGF-β + Hh-activated P = 0.0003; Grem1: Control v TGF-β P = 0.4845, Control v Hh-activated P = 0.2422, Control v TGF-β + Hh-activated P = 0.1417; Fzd1: Control v TGF-β P = 0.3341, Control v Hh-activated P = 0.2689, Control v TGF-β + Hh-activated P = 0.3320; Sfrp1: Control v TGF-β P = 0.4103, Control v Hh-activated P = 0.4614, Control v TGF-β + Hh-activated P = 0.1093; ordinary one-way ANOVA test. Data are expressed as mean ± SD. Scale bars, 100 µm.