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. Author manuscript; available in PMC: 2021 Apr 12.
Published in final edited form as: Nat Cell Biol. 2020 Oct 12;22(11):1295–1306. doi: 10.1038/s41556-020-00591-9

Figure 6. BMP activation attenuates metaplastic airway progenitor differentiation.

Figure 6.

(a-c) Histology quantification of areas of KRT5+ pods and percentage of SFTPC+ cells and oximetry after addition of rhBMP4 during bleomycin injury (n = 8 per group; each data point represents one animal; one-tailed unpaired Student’s t-test for (b,c)). Data are expressed as mean ± SD.

(d,e) Histologic quantification of alveolar Sox2 Lin+ cells followed by bleomycin injury and rhBMP4 administration (n = 4 per group; each data point represents one animal; one-tailed unpaired Student’s t-test). Data are expressed as mean ± SD.

(f,g) qPCR of epithelial organoids co-cultured with Hh-activated mesenchyme and rhBMP4. (n = 6 for vehicle, n = 6 for Hh-activated, and n = 5 for Hh-activated + BMP4; each data point represents one well; ordinary one-way ANOVA test). Data are expressed as mean ± SD.

(h) Model of BMP activation promoting airway progenitor differentiation into adaptive SFTPC+ Type 2 cells.

AW = airway, Scale bars, 100 µm.