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. Author manuscript; available in PMC: 2021 Apr 12.
Published in final edited form as: Nat Cell Biol. 2020 Oct 12;22(11):1295–1306. doi: 10.1038/s41556-020-00591-9

Extended Data Fig. 1. Epithelial progenitors expand distally in fibrotic repair.

Extended Data Fig. 1

(a,b) Time course showing appearance of metaplastic airway epithelium in the alveoli after 1, 2, 3 or 4 doses of weekly bleomycin injury. (n = 3 per group; each data point represents one animal). Data are expressed as mean ± SD.

(c,d) Contribution of Sox2 Lin+ cells to the various epithelial lineages in the alveoli after bleomycin injury. (n = 6 per group; each data point represents one animal; one-tailed unpaired Student’s t-test). Data are expressed as mean ± SD.

(e) Lack of Sox2 Lin+ cells in corn oil-treated SoxcreERT2:R26mTmG lung. (2 animals)

(f) Co-localization of Sox2 Lin+ cells and SOX2 staining. (n = 3 animals). Data are expressed as mean ± SD.

(g) Extended washout after tamoxifen (>4wks) followed by bleomycin demonstrates similar expansion of Sox2 Lin+ cells into the alveoli (3 animals).

AW = airway. Scale bars, 100 µm.