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. Author manuscript; available in PMC: 2021 Apr 12.
Published in final edited form as: Nat Biotechnol. 2020 Oct 12;38(11):1274–1279. doi: 10.1038/s41587-020-0703-0

Figure 2. The GRF4-GIF1 chimera induces embryogenesis in the absence of cytokinins.

Figure 2.

A) Schematic representation of the different steps of wheat transformation. B). Representative calli in auxin media with no hygromycin. Note growing green shoots in callus transformed with the wheat GRF4-GIF1 chimera in the absence of cytokinins (red arrows). Control: pLC41. C) Transgenic specific PCR product (yellow arrow) amplified with primers pLC41–1064 and pLC41–1061 (Supplementary Table S1). In this first experiment (out of three), we identified five transgenic plants among nine regenerated from the GRF4-GIF1 marker-free vector and no transgenic plants among four regenerated from the control.