Glial metabolic rewiring promotes axon regeneration and functional recovery in the central nervous system

. Author manuscript; available in PMC: 2021 Nov 3.

Published in final edited form as: Cell Metab. 2020 Sep 16;32(5):767–785.e7. doi: 10.1016/j.cmet.2020.08.015

KEY RESOURCES TABLE

REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Mouse anti-GFAP	Sigma Aldrich	Cat#G3893; RRID:AB_477010
Rabbit anti-ALDH1L1	Abcam	Cat#ab87117; RRID:AB_10712968
Mouse anti-GABA B Receptor 1	Abcam	Cat#ab55051; RRID:AB_941703
Rabbit anti-GABA B Receptor 2	Novus Biologicals	Cat#NBP2-16569
Bacterial and Virus Strains



Biological Samples



Chemicals, Peptides, and Recombinant Proteins
Sodium L-lactate	Sigma Aldrich	Cat#71718
Ethyl L-lactate	Sigma Aldrich	Cat#69799
Disodium L-2-Hydroxyglutarate	Sigma Aldrich	Cat#90790
octyl-L-2HG	Cayman Chemical	Cat#1391194-64-1
L-2-Hydroxybutyric acid	Sigma Aldrich	Cat#54918
Ethyl 2-Hydroxybutyrate	Fisher Scientific	Cat#H02295G
L-tartaric acid	Sigma Aldrich	Cat#251380
Diethyl L-tartrate	Sigma Aldrich	Cat#W237809
Critical Commercial Assays
Quick-RNA MiniPrep Plus Kit	Zymo Research	Cat#R1058
TSA Plus Cyanine 3 System	PerkinElmer	Cat#NEL744B001KT
Deposited Data
Raw and analyzed RNA-seq data	This paper	GEO: GSE144655
Experimental Models: Cell Lines



Experimental Models: Organisms/Strains
D. melanogaster: ppk-CD4-tdGFP	Han et al., 2011	N/A
D. melanogaster: ppk-CD4-tdTomato	Han et al., 2011	N/A
D. melanogaster: ppk-Gal4	Grueber et al., 2003	N/A
D. melanogaster: repo-Gal4	Sepp et al., 2001	N/A
D. melanogaster: UAS-Egfr^CA (dEGFR^λ)	Read et al., 2009	N/A
D. melanogaster: UAS-Pi3K92E^CA (Dp110^CAAX)	Read et al., 2009	N/A
D. melanogaster: alrm-Gal4	Doherty et al., 2009	N/A
D. melanogaster: TIFR-Gal4	Ziegenfuss et al., 2012	N/A
D. melanogaster: Ldh¹⁶	Li et al., 2017	N/A
D. melanogaster: Ldh¹⁷	Li et al., 2017	N/A
D. melanogaster: L2HGDH¹²	Li et al., 2017	N/A
D. melanogaster: L2HGDH¹⁴	Li et al., 2017	N/A
D. melanogaster: UAS-L2HGDH	Li et al., 2017	N/A
D. melanogaster: UAS-cAMPr	Hackley et al., 2018	N/A
D. melanogaster: GMR54H02-Gal4: w¹¹¹⁸; P{GMR54H02-GAL4}attP2	Bloomington Drosophilia Stock Center	BDSC: 45784
D. melanogaster: repo-QF: y¹ w; Pin1/CyO; P{ET-QF2.GU}repo/TM6B, Tb1*	Bloomington Drosophilia Stock Center	BDSC: 66477
D. melanogaster: UAS-btl^CA: w; P{UAS-btl.λ}2*	Bloomington Drosophilia Stock Center	BDSC: 29045
D. melanogaster: UAS-htl^CA: y¹ w; P{UAS-htl.λ.M}40-22-2*	Bloomington Drosophilia Stock Center	BDSC: 5367
D. melanogaster: UAS-stg: w; P{UAS-stg.HA}2*	Bloomington Drosophilia Stock Center	BDSC: 56562
D. melanogaster: UAS-sima: w; P{UAS-sima.B}2*	Bloomington Drosophilia Stock Center	BDSC: 9582
D. melanogaster: UAS-ci: w; P{UAS-ci.HA.wt}3*	Bloomington Drosophilia Stock Center	BDSC: 32570
D. melanogaster: UAS-foxo: w¹¹¹⁸; P{UASp-foxo.S}3	Bloomington Drosophilia Stock Center	BDSC: 42221
D. melanogaster: UAS-p53.H159N: y¹ w¹¹¹⁸; P{UAS-p53.H159N.Ex}2	Bloomington Drosophilia Stock Center	BDSC: 8420
D. melanogaster: Myc⁴: Myc4/FM7i, P{ActGFP}JMR3	Bloomington Drosophilia Stock Center	BDSC: 64769
D. melanogaster: UAS-mCherry: y¹ sc v¹ sev²¹; P{UAS-mCherry.VALIUM10}attP2*	Bloomington Drosophilia Stock Center	BDSC: 35787
D. melanogaster: UAS-Myc: w¹¹¹⁸; P{UAS-Myc.Z}132	Bloomington Drosophilia Stock Center	BDSC: 9674
D. melanogaster: QUAS-Myc: y¹ w¹¹¹⁸; P{QUAS-Myc.P}7/CyO	Bloomington Drosophilia Stock Center	BDSC: 30009
D. melanogaster: UAS-IdhR195H: w¹¹¹⁸; P{UAS-Idh.R195H.FLAG}3	Bloomington Drosophilia Stock Center	BDSC: 56203
D. melanogaster: GABA-B-R1^MI03255: y¹ w; Mi{MIC}GABA-B-R1^MI03255/SM6a*	Bloomington Drosophilia Stock Center	BDSC: 36226
D. melanogaster: GABA-B-R1^{MI01930-GFSTF.0}: y¹ w^67c23; Mi{PT-GFSTF.0}GABA-B-R1^{MI01930-GFSTF.0}	Bloomington Drosophilia Stock Center	BDSC: 60522
D. melanogaster: Ldh RNAi#2: y¹ v¹; P{TRiP.HMS00039}attP2	Bloomington Drosophilia Stock Center	BDSC: 33640
D. melanogaster: UAS-sima RNAi#2: y¹ sc v¹ sev²¹; P{TRiP.HMS00832}attP2*	Bloomington Drosophilia Stock Center	BDSC: 33894
D. melanogaster: MESK2 RNAi: y¹ v¹; P{TRiP.JF03312}attP2	Bloomington Drosophilia Stock Center	BDSC: 29380
D. melanogaster: L2HGDH RNAi#2: y¹ v¹; P{TRiP.HMC03444}attP40	Bloomington Drosophilia Stock Center	BDSC: 51870
D. melanogaster: UAS-GABA-B-R1 RNAi#1: y¹ v¹; P{TRiP.HMC03388}attP2	Bloomington Drosophilia Stock Center	BDSC: 51817
D. melanogaster: Myc* RNAi#1: w¹¹¹⁸; P{GD1419}v2947*	VDRC Stock Center	VDRC: v2947
D. melanogaster: Myc RNAi#2: P{KK103869}VIE-260B	VDRC Stock Center	VDRC: v106066
D. melanogaster: Ldh RNAi#1: w¹¹¹⁸; P{GD6887}v31192/TM3	VDRC Stock Center	VDRC: v31192
D. melanogaster: HexA RNAi#1: w¹¹¹⁸; P{GD9964}v21054	VDRC Stock Center	VDRC: v21054
D. melanogaster: HexA RNAi#2: P{KK100831}VIE-260B	VDRC Stock Center	VDRC: v104680
D. melanogaster: Pfk RNAi#1: w¹¹¹⁸; P{GD1508}v3016	VDRC Stock Center	VDRC: v3016
D. melanogaster: Pfk RNAi#2: P{KK101887}VIE-260B	VDRC Stock Center	VDRC: v105666
D. melanogaster: Pdha* RNAi#1: w¹¹¹⁸; P{GD12103}v40410*	VDRC Stock Center	VDRC: v40410
D. melanogaster: Pdha* RNAi#2: P{KK101856}VIE-260B*	VDRC Stock Center	VDRC: v107209
D. melanogaster: muc RNAi#1: P{GD16843}v48941	VDRC Stock Center	VDRC: v48941
D. melanogaster: muc RNAi#2: P{KK102893}VIE-260B	VDRC Stock Center	VDRC: v110735
D. melanogaster: sima RNAi#1: P{KK102226}VIE-260B	VDRC Stock Center	VDRC: v106187
D. melanogaster: Mpc1 RNAi#1: w¹¹¹⁸; P{GD3944}v15858	VDRC Stock Center	VDRC: v15858
D. melanogaster: Mpc1 RNAi#2: P{KK102734}VIE-260B	VDRC Stock Center	VDRC: v103829
D. melanogaster: CG9399 RNAi: P{KK109212}VIE-260B	VDRC Stock Center	VDRC: v101455
D. melanogaster: CG9396 RNAi: P{KK112662}VIE-260B	VDRC Stock Center	VDRC: v104068
D. melanogaster: L2HGDH RNAi#1: w¹¹¹⁸; P{GD5322}v30737	VDRC Stock Center	VDRC: v30737
D. melanogaster: Sln RNAi#1: w¹¹¹⁸; P{GD1940}v4607	VDRC Stock Center	VDRC: v4607
D. melanogaster: Sln RNAi#2: P{KK104306}VIE-260B	VDRC Stock Center	VDRC: v109464
D. melanogaster: out RNAi#1: w¹¹¹⁸; P{GD3448}v51157	VDRC Stock Center	VDRC: v51157
D. melanogaster: out RNAi#2: P{KK104187}VIE-260B	VDRC Stock Center	VDRC: v108364
D. melanogaster: GABA-B-R3 RNAi: P{KK105961}VIE-260B	VDRC Stock Center	VDRC: v108036
D. melanogaster: CG2082 RNAi: w¹¹¹⁸; P{GD8635}v19120	VDRC Stock Center	VDRC: v19120
D. melanogaster: Rdl RNAi: P{KK104293}VIE-260B	VDRC Stock Center	VDRC: v100429
D. melanogaster: GABA-B-R1 RNAi#2: P{VSH330042}attP40	VDRC Stock Center	VDRC: v330042
D. melanogaster: GABA-B-R2 RNAi#1: w¹¹¹⁸; P{GD699}v1784	VDRC Stock Center	VDRC: v1784
D. melanogaster: GABA-B-R2 RNAi#2: P{KK100020}VIE-260B	VDRC Stock Center	VDRC: v110268
D. melanogaster: UAS-Grn: M{UAS-grn.ORF.3xHA.GW}ZH-86Fb	FlyORF	FlyORF: F001916
D. melanogaster: UAS-Smox: M{UAS-Smox.ORF.3xHA.GW}ZH-86Fb	FlyORF	FlyORF: F000025
D. melanogaster: UAS-Su(H): M{UAS-Su(H).ORF.3xHA.GW}ZH-86Fb	FlyORF	FlyORF: F001922
D. melanogaster: UAS-Stat92E: M{UAS-Stat92E.ORF.3xHA}ZH-86Fb	FlyORF	FlyORF: F000750
D. melanogaster: UAS-Ldh: M{UAS-Ldh.ORF.3xHA.GW}ZH-86Fb	FlyORF	FlyORF: F002924
D. melanogaster: Pdha^G0334: y¹ w P{lacW}PdhaG0334 P{neoFRT}19A/FM7c; P{ey-FLP.N}5*	Kyoto Stock Center	DGRC: 111869
D. melanogaster: UAS-Laconic	This paper	N/A
Mouse: C57BL/6J	The Jackson Laboratory	IMSR_JAX:000664
Oligonucleotides
GABA-B-R1 forward primer: cgcttccctcatcaactcatc	This paper	N/A
GABA-B-R1 reverse primer: gtataattcacctatcacgcaaagg	This paper	N/A
rp49 forward primer: cagtcggatcgatatgctaagctg	This paper	N/A
rp49 reverse primer: taaccgatgttgggcatcagatac	This paper	N/A
Recombinant DNA
plasmid: UAS-Laconic	This paper	N/A
Software and Algorithms
ImageJ	https://imagej.nih.gov/ij/	N/A
GraphPad Prism 7	GraphPad software	N/A
Zen	Zeiss	N/A
Photoshop	Adobe	N/A
Illustrator	Adobe	N/A
Microsoft Excel 2016	Microsoft Corporation	N/A
Heatmapper	http://www.heatmapper.ca/	N/A
Metascape	https://metascape.org/gp/index.html#/main/step1	N/A
Superheat	https://rlbarter.github.io/superheat/	N/A
Other