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Isolation and characterization of stem/progenitor spermatogonia. a Flow cytometric analysis of stem/progenitor spermatogonia for CD90.2, PLZF, and c-kit antibodies from whole testis after enzymatic digestion and after MACS separation. b Bright-field phase contrast micrographs (low × 100 and high × 200 magnification) and IF labeling of cultured stem/progenitor spermatogonia for PLZF and Ob-Rb leptin receptor. DAPI; nucleus. × 600
